TY - JOUR T1 - Radiolabelled ketone body uptake in prostate tumor cells is mediated by fatty acid synthesis JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1584 LP - 1584 VL - 50 IS - supplement 2 AU - Simon Authier AU - Sebastien Tremblay AU - Stephen Cunnane AU - Francois Benard Y1 - 2009/05/01 UR - http://jnm.snmjournals.org/content/50/supplement_2/1584.abstract N2 - 1584 Objectives Cancer cells can utilize ketone bodies as alternate fuels to meet their requirements. Previous studies have shown the potential to use 11C-acetoacetate, a ketone body, for PET imaging of cancer. The objective of this study was to determine the impact of fatty acid synthesis on 11C-acetoacetate uptake by breast and prostate tumors. Methods Murine mammary cancer cells (MC7-L1, MC4-L2), and human prostate cancer cells (PC-3, LN-CaP) were seeded in 6-well plates 24 hours before the experiment. 30μM of 5-(tetradecyloxy)-2-furoic acid (TOFA, a fatty acid synthesis inhibitor) in dimethyl sulfoxide (DMSO) was added to the growth media. Control cells received vehicle only. After a 2 hours incubation at 37°C, 11C-acetoacetate was added to the each well. After different uptake times, the cells were washed and tracer incorporation was measured. The results were expressed as %I.D./µg of protein. Results Control cells showed progressive tracer uptake over time. The control tumor cell uptake was consistently higher than the uptake of cells in contact with TOFA. For the breast cancer cell lines, a maximal inhibition of 21,3 ± 2,85% (MC7-L1) and 16,0 ± 5,13% (MC4-L2) at 15 min did not reach statistical significance. For the prostate cancer cell lines, the presence of TOFA significantly reduced 11C-acetoacetate incorporation. The maximal inhibition was reached at 60 min for the PC-3 (27,1 ± 0,29% P = 0,0016) and at 30 min for the LN-CaP (39,1 ± 0,30%, P = 0,0003). Conclusions Our results show the importance of fatty acid synthesis in the uptake of 11C-acetoacetate by prostate cancer cells. ER -