TY - JOUR T1 - Imaging colon cancer response to treatment with AZD1152 with [18F] fluoro-2-deoxyglucose and 3'-deoxy-3'-[18F] fluorothymidine JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1602 LP - 1602 VL - 50 IS - supplement 2 AU - Maxim Moroz AU - Elisa De Stanchina AU - Inna Serganova AU - Gary Schwartz AU - Ronald Blasberg Y1 - 2009/05/01 UR - http://jnm.snmjournals.org/content/50/supplement_2/1602.abstract N2 - 1602 Objectives The objective of the study was to assess effect of an Aurora Kinase B inhibitor, AZD1152, on colon cancer xenografts and to determine whether treatment response could be monitored by non-invasive [18F]FDG and/or [18F]FLT PET imaging. Methods Experimental cell lines SW620 and HCT116 were acquired from ATCC. Xenografts were established by subcutaneous injection of 1 x 106 cells in the right flank of Rnu/rnu mice (NCI, MD). SW620 or HCT116 xenografts were given ip injections (100 mg/kg) of AZD1152 daily on two consecutive days per week over a 3 week interval. Mice were imaged on a R4 MicroPET (Concorde, Knoxville, TN) following iv injections of [18F]FDG or [18F]FLT on consecutive days. Results A treatment response based on tumor volume measurements was clearly obtained in both HCT116 and SW620 xenografts. [18F]FDG PET imaging showed no difference in glucose utilization between AZD1152-treated and non-treated HCT116 or SW620 xenografts. SW620 xenografts showed no accumulation of [18F]FLT and there was no differences in [18F]FLT accumulation between AZD1152-treated and non-treated SW620 xenografts. [18F]FLT uptake in untreated HCT116 xenografts was more than 4–fold greater than that in AZD1152-treated HCT116 xenografts. Conclusions AZD1152 treatment was effective in two different xenograft models based on sequential tumor volume measurements. However, [18F]FDG PET was not a useful imaging paradigm for monitoring of AZD1152 treatment response. [18F]FLT PET was effective in monitoring treatment response in HCT116 xenografts, but not in SW620 xenografts. The latter observation further illustrates that [18F]FLT is not an effective cell proliferation tracer in some tumors. ER -