RT Journal Article
SR Electronic
T1 Imaging fibrosarcoma with Tc-99m labeled polymer pretargeted with bombesin-bispecific antibody complex
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1607
OP 1607
VO 50
IS supplement 2
A1 Ban-An  Khaw
A1 Vishwesh Patil
A1 David Pincus
A1 Alexandra  Varvarigou
A1 Stan Majewski
A1 Alexander  Stolin
A1 Yared Tekabe
YR 2009
UL http://jnm.snmjournals.org/content/50/supplement_2/1607.abstract
AB 1607 Objectives Whether murine Fibrosarcoma can be imaged with Tc-99m DTPA-polymers(Tc-DSPL) after pretargeting with Bombesin-anti-DTPA bispecific antibody complex (BABAC) was studied. Methods 105 MCA205 cells were injected sc in the shoulder of mice. After 14 days 10 μg BABAC (n = 3) or saline (n = 4) were injected iv. Then 37 MBq Tc-DSPL were injected iv 24 h later. Mice were imaged at injection, 10 -15 minutes and 24 hours. Each image was analyzed by computer planimetry for tumor, and contralateral shoulder, region activities. Tumor to contralateral pixel density ratios were correlated to time of imaging. Results Tumors pretargeted with BABAC can be visualized in 15 min. Mean uptake ratio at 24 hours (6.7+0.84) was significantly greater than that of control mice (2.16+1.45 , p < 0.01). An increase in the uptake ratio of Tc-DSPL in tumors pre-targeted with BABAC (y = 0.86Ln(x) +4.001, R2= 0.98) was observed, whereas control mice injected showed a correlation of y = 0.13Ln(x) +1.68 (R2 = 0.593). Conclusions BABAC can be used to pre-target MCA205 Fibrosarcoma in mice with Tc-DSPL. Although Tc-DSPL localized in Fibrosacroma via “Enhanced Permeability Retention (EPR) ” effect at 24 h, uptake of Tc-DSPL in Fibrosarcoma pretargeted with BABAC was approximately 3 times greater.