@article {Zhou158P, author = {Yun Zhou and Michael Weed and Ming-Kai Chen and Arman Rahmim and Weiguo Ye and James Bra{\v s}i\#263; and Mohab Alexander and Andrew Crabb and Jennifer McGlothan and Farah Ali and Tom{\'a}s Guilarte and Dean Wong}, title = {Quantitative dopamine transporter imaging studies in nonhuman primates with a GE Advance and high resolution research tomography (HRRT) PET scanners}, volume = {48}, number = {supplement 2}, pages = {158P--158P}, year = {2007}, publisher = {Society of Nuclear Medicine}, abstract = {541 Objectives: To quantify [11C]methylphenidate ([11C]MP) striatum binding in monkeys with a GE Advance and high resolution research tomography (HRRT) PET scanners. Methods: Dopamine transporter imaging using [11C]MP was performed on two groups: 21 Rhesus monkeys (body weight 3-5 kg) scanned on HRRT; and 24 cynomologous monkeys (body weight 5-9 kg) scanned on GE Advance. A bolus of [11C]MP was injected intravenously and dynamic images of 30 frames were reconstructed for 90 min data acquisition in each study. The measured striatum time activity curve (TAC) was analyzed by two reference tissue methods using cerebellum TAC as input: a simplified reference tissue model (SRTM) (Lammertsam and Hume, 1996, Neuroimage 4: 153-158); and graphical analysis (GA) (Logan et al., 1996, JCBFM 16: 834-840). The BP estimated by the GA as a function of the population mean of k2R (the efflux rate constant of [11C]MP in cerebellum) in [0.02 0.2] were generated. Results: The estimates of (R1(the transport rate ratio of striatum to cerebellum), k2(the efflux rate constant in striatum), BP (binding potential), and k2R (calculated as k2/R1 after model fitting)) (mean {\textpm} SD) from the SRTM were (1.114 {\textpm} 0.135, 0.072 {\textpm} 0.020, 2.811 {\textpm} 0.343, 0.064 {\textpm} 0.015 ) for Rhesus monkeys with HRRT scanner and (0.938 {\textpm} 0.109, 0.036 {\textpm} 0.007, 1.852 {\textpm} 0.363, 0.039 {\textpm} 0.008 ) for cynomologous monkeys with GE Advance scanner. The BP estimates from GA decreased monotonically in k2R. The BP estimates from GA were not significantly different from those obtained by SRTM for the given k2R in [0.053 0.063] in HRRT group study and k2R in [0.035 0.039] in GE Advance group. Conclusions: The parameters of R1, k2, BP, and k2R estimated from the SRTM method for the Rhesus monkeys with HRRT scanner studies were significantly higher than those from the cynomologous monkeys with GE Advance scanner. The [11C]MP BP estimates from the GA approach is sensitive to the pre-determined population mean of k2R. Research Support (if any): Grant support AA12839, DA00412, NS38927, MH075378}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/48/supplement_2/158P.3}, eprint = {https://jnm.snmjournals.org/content}, journal = {Journal of Nuclear Medicine} }