RT Journal Article SR Electronic T1 Late Imaging with [1-11C]Acetate Improves Detection of Tumor Fatty Acid Synthesis with PET JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 1144 OP 1149 DO 10.2967/jnumed.113.134437 VO 55 IS 7 A1 David Y. Lewis A1 Joan Boren A1 Greg L. Shaw A1 Robert Bielik A1 Antonio Ramos-Montoya A1 Timothy J. Larkin A1 Carla P. Martins A1 David E. Neal A1 Dmitry Soloviev A1 Kevin M. Brindle YR 2014 UL http://jnm.snmjournals.org/content/55/7/1144.abstract AB Tumors are often characterized by high levels of de novo fatty acid synthesis. The kinetics of acetate incorporation into tricarboxylic acid cycle intermediates and into lipids suggest that detection of tumors with [1-11C]acetate PET could be improved by imaging at later time points. Methods: The uptake and metabolism of [1-11C], [1-13C], and [1-14C]acetate were measured in mouse prostate and lung cancer models to investigate the time course of 11C label incorporation into tumor metabolites. Results: Radioactivity in the lipid fraction, as compared with the aqueous fraction, in extracts of C4-2B human prostate xenografts peaked at 90 min after [1-14C]acetate injection, which coincided with peak 13C label incorporation into the fatty acids palmitate and stearate. Contrast between the tumor and tissues, such as blood and muscle, increased in PET images acquired over a period of 120 min after [1-11C]acetate injection, and Patlak plots were linear from 17.5 min after injection. Similar results were obtained in a genetically engineered K-rasG12D; p53null lung cancer model, in which the mean tumor-to-lung ratio at 90 min after [1-14C]acetate injection was 4.4-fold higher than at 15 min. Conclusion: These findings suggest that when imaging de novo fatty acid synthesis with [1-11C]acetate it is preferable to measure uptake at later time points, when the effects of perfusion and 11C incorporation into tricarboxylic acid cycle intermediates and bicarbonate are declining. The data presented here suggest that future clinical PET scans of tumors should be acquired later than 30 min, when tracer accumulation due to de novo fatty acid synthesis prevails.