TY - JOUR T1 - Multimodal Molecular Imaging of Integrin α<sub>v</sub>β<sub>3</sub> for In Vivo Detection of Pancreatic Cancer JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 446 LP - 451 DO - 10.2967/jnumed.113.129619 VL - 55 IS - 3 AU - Marija Trajkovic-Arsic AU - Pouyan Mohajerani AU - Athanasios Sarantopoulos AU - Evdokia Kalideris AU - Katja Steiger AU - Irene Esposito AU - Xiaopeng Ma AU - George Themelis AU - Neal Burton AU - Christoph W. Michalski AU - Jörg Kleeff AU - Stefan Stangl AU - Ambros J. Beer AU - Karolin Pohle AU - Hans-Jürgen Wester AU - Roland M. Schmid AU - Rickmer Braren AU - Vasilis Ntziachristos AU - Jens T. Siveke Y1 - 2014/03/01 UR - http://jnm.snmjournals.org/content/55/3/446.abstract N2 - Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease. Late detection of then nonresectable or metastasized tumors emphasizes the need for novel imaging approaches. Here, we report on so far nonexploited potentials of αvβ3 integrin–targeted molecular imaging technologies for detection of PDAC using genetically engineered mouse models. Methods: Immunohistochemistry and Western blot were used for characterization of αvβ3 expression in murine and human PDAC. We applied IntegriSense 680 fluorescence molecular tomography, intraoperative fluorescence imaging, and 68Ga-NODAGA-RGD PET for αvβ3 integrin molecular in vivo imaging of spontaneous PDAC occurring in Ptf1a+/Cre;Kras+/LSL-G12D;p53LoxP/LoxP mice. (NODAGA is 1,4,7-triazacyclononane-1,4-bis[acetic acid]-7-[2-glutaric acid] and RGD is arginine-glycine-aspartic acid.) Results: αvβ3 integrin is expressed in tumor cells of human and murine PDAC. IntegriSense fluorescence molecular tomography and 68Ga-NODAGA-RGD PET enabled faithful visualization of PDAC. Furthermore, intraoperative optical imaging with IntegriSense 680 allowed good delineation of tumor borders. Conclusion: Imaging approaches targeting αvβ3 integrin expand the potential of molecular imaging for identification of αvβ3-positive PDAC with potential implications in early detection, fluorescence-guided surgery, and therapy monitoring. ER -