RT Journal Article
SR Electronic
T1 Preclinical Evaluation of <sup>18</sup>F-LMI1195 for In Vivo Imaging of Pheochromocytoma in the MENX Tumor Model
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 2111
OP 2117
DO 10.2967/jnumed.113.119966
VO 54
IS 12
A1 Florian C. Gaertner
A1 Tobias Wiedemann
A1 Behrooz H. Yousefi
A1 Misu Lee
A1 Ines Repokis
A1 Takahiro Higuchi
A1 Stephan G. Nekolla
A1 Ming Yu
A1 Simon Robinson
A1 Markus Schwaiger
A1 Natalia S. Pellegata
YR 2013
UL http://jnm.snmjournals.org/content/54/12/2111.abstract
AB We evaluated 18F-LMI1195 (1-(3-bromo-4-(3-18F-fluoro-propoxy)benzyl)guanidine), a metaiodobenzylguanidine (MIBG) analog, for the detection of pheochromocytoma in a preclinical in vivo model of endogenous neuroendocrine tumors (multiple endocrine neoplasia [MENX]). Methods: Adrenal uptake kinetics of 18F-LMI1195 were evaluated in healthy Wistar rats (n = 6) by dynamic PET imaging. Distribution of 18F-LMI1195 was evaluated in tumor-bearing MENX mut/mut rats (n = 10) and control MENX wild-type rats (n = 4) by biodistribution studies and PET imaging. Biodistribution of 18F-LMI1195 was compared with 123I-MIBG in MENX mut/mut rats (n = 6) and correlated with histological tumor volume and norepinephrine transporter (NET) expression. Uptake specificity was evaluated by in vivo inhibition of the NET by desipramine (n = 6). Intraadrenal distribution of 18F-LMI1195 was evaluated by autoradiography. Results: 18F-LMI1195 showed rapid tracer accumulation in adrenal glands 1 min after tracer injection. Adrenal glands of MENX mut/mut animals showed significantly higher standardized uptake value than MENX wild-type controls (maximum SUV, 10.3 ± 2.3 vs. 6.1 ± 0.9, P &lt; 0.01). Adrenal uptake in MENX mut/mut rats could be inhibited by desipramine, shown by biodistribution studies (0.06 ± 0.01 vs. 0.16 ± 0.05 percentage injected dose, P &lt; 0.01), PET imaging (maximum SUV, 3.8 ± 0.8 vs. 10.3 ± 2.3, P &lt; 0.01), and autoradiography. Adrenal uptake of 18F-LMI1195 correlated with 123I-MIBG uptake (r = 0.91), histological tumor volume (r = 0.68), and NET expression (r = 0.50). 18F-LMI1195 showed an overall favorable distribution for tumor imaging. Conclusion: 18F-LMI1195 shows high and specific accumulation in pheochromocytomas. Its favorable biodistribution makes it a promising PET tracer for tumor imaging. Further studies are warranted to evaluate its clinical value in oncologic indications.