PT  - JOURNAL ARTICLE
AU  - Jun Wang
AU  - Kiwook Hwang
AU  - Daniel Braas
AU  - Alex Dooraghi
AU  - David Nathanson
AU  - Dean O. Campbell
AU  - Yuchao Gu
AU  - Troy Sandberg
AU  - Paul Mischel
AU  - Caius Radu
AU  - Arion F. Chatziioannou
AU  - Michael E. Phelps
AU  - Heather Christofk
AU  - James R. Heath
TI  - Fast Metabolic Response to Drug Intervention Through Analysis on a Miniaturized, Highly Integrated Molecular Imaging System
AID  - 10.2967/jnumed.112.118497
DP  - 2013 Oct 01
TA  - Journal of Nuclear Medicine
PG  - 1820--1824
VI  - 54
IP  - 10
4099  - http://jnm.snmjournals.org/content/54/10/1820.short
4100  - http://jnm.snmjournals.org/content/54/10/1820.full
SO  - J Nucl Med2013 Oct 01; 54
AB  - We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs. Methods: A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to 18F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling. Results: The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis. Conclusion: The kinetic analysis enabled by the platform provided a rapid (∼1 h) drug screening assay.