RT Journal Article
SR Electronic
T1 Molecular Imaging of Conscious, Unrestrained Mice with AwakeSPECT
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 969
OP 976
DO 10.2967/jnumed.112.109090
VO 54
IS 6
A1 Justin S. Baba
A1 Christopher J. Endres
A1 Catherine A. Foss
A1 Sridhar Nimmagadda
A1 Hyeyun Jung
A1 James S. Goddard
A1 Seungjoon Lee
A1 John McKisson
A1 Mark F. Smith
A1 Alexander V. Stolin
A1 Andrew G. Weisenberger
A1 Martin G. Pomper
YR 2013
UL http://jnm.snmjournals.org/content/54/6/969.abstract
AB We have developed a SPECT imaging system, AwakeSPECT, to enable molecular brain imaging of untrained mice that are conscious, unanesthetized, and unrestrained. We accomplished this with head tracking and motion correction techniques. Methods: The capability of the system for motion-corrected imaging was demonstrated with a 99mTc-pertechnetate phantom, 99mTc-methylene diphosphonate bone imaging, and measurement of the binding potential of the dopamine transporter radioligand 123I-ioflupane in mouse brain in the awake and anesthetized (isoflurane) states. Stress induced by imaging in the awake state was assessed through measurement of plasma corticosterone levels. Results: AwakeSPECT provided high-resolution bone images reminiscent of those obtained from CT. The binding potential of 123I-ioflupane in the awake state was on the order of 50% of that obtained with the animal under anesthesia, consistent with previous studies in nonhuman primates. Levels of stress induced were on the order of those seen in other behavioral tasks and imaging studies of awake animals. Conclusion: These results demonstrate the feasibility of SPECT molecular brain imaging of mice in the conscious, unrestrained state and demonstrate the effects of isoflurane anesthesia on radiotracer uptake.