PT  - JOURNAL ARTICLE
AU  - Yoshimoto, Mitsuyoshi
AU  - Hayakawa, Takuya
AU  - Mutoh, Michihiro
AU  - Imai, Toshio
AU  - Tsuda, Keisuke
AU  - Kimura, Sadaaki
AU  - Umeda, Izumi O.
AU  - Fujii, Hirofumi
AU  - Wakabayashi, Keiji
TI  - In Vivo SPECT Imaging with <sup>111</sup>In-DOTA-c(RGDfK) to Detect Early Pancreatic Cancer in a Hamster Pancreatic Carcinogenesis Model
AID  - 10.2967/jnumed.111.099630
DP  - 2012 May 01
TA  - Journal of Nuclear Medicine
PG  - 765--771
VI  - 53
IP  - 5
4099  - http://jnm.snmjournals.org/content/53/5/765.short
4100  - http://jnm.snmjournals.org/content/53/5/765.full
SO  - J Nucl Med2012 May 01; 53
AB  - Early detection of pancreatic cancer is key to overcoming its poor prognosis. αvβ3-integrin is often overexpressed in pancreatic tumor cells, whereas it is scarcely expressed in normal pancreatic cells. In this study, we investigated the usefulness of SPECT imaging with 111In-1,4,7,10-tetraazacylododecane-N,N′,N″,N′′′-tetraacetic acid-cyclo-(Arg-Gly-Asp-d-Phe-Lys) [111In-DOTA-c(RGDfK)], an imaging probe of αvβ3-integrin, for the early detection of pancreatic cancer in a hamster pancreatic carcinogenesis model. Methods: Hamsters were subcutaneously injected with the pancreatic duct carcinogen N-nitrosobis(2-oxopropyl)amine to induce pancreatic cancer. N-nitrosobis(2-oxopropyl)amine–treated hamsters underwent in vivo SPECT with 111In-DOTA-c(RGDfK). After imaging, the tumor-to-normal pancreatic tissue radioactivity ratios in excised pancreatic samples were measured with autoradiography (ARG) and compared with the immunopathologic findings for αvβ3-integrin. In a mouse model in which inflammation was induced with turpentine, the uptake of 111In-DOTA-c(RGDfK) in inflammatory regions was evaluated with ARG and compared with that of 18F-FDG. Results: 111In-DOTA-c(RGDfK) was clearly visualized in pancreatic cancer lesions as small as 3 mm in diameter. ARG analysis revealed high tumor-to-normal pancreatic tissue radioactivity ratios (4.6 ± 1.0 [mean ± SD] in adenocarcinoma and 3.3 ± 1.4 in atypical hyperplasia). The uptake of 111In-DOTA-c(RGDfK) strongly correlated with αvβ3-integrin expression. In the inflammatory model, inflammation-to-muscle ratios for 18F-FDG and 111In-DOTA-c(RGDfK) were 8.37 ± 4.37 and 1.98 ± 0.60, respectively. These results imply that 111In-DOTA-c(RGDfK) has a lower rate of false-positive tumor detection than 18F-FDG. Conclusion: Our findings suggest that SPECT with 111In-DOTA-c(RGDfK) has great potential for the early and accurate detection of pancreatic cancer.