RT Journal Article
SR Electronic
T1 Nanoparticles Modified by Encapsulation of Ligands with a Long Alkyl Chain to Affect Multispecific and Multimodal Imaging
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1462
OP 1470
DO 10.2967/jnumed.111.092759
VO 53
IS 9
A1 Young Kyoung Lee
A1 Jae Min Jeong
A1 Lathika Hoigebazar
A1 Bo Yeun Yang
A1 Yun-Sang Lee
A1 Byung Chul Lee
A1 Hyewon Youn
A1 Dong Soo Lee
A1 June-Key Chung
A1 Myung Chul Lee
YR 2012
UL http://jnm.snmjournals.org/content/53/9/1462.abstract
AB The attachment of specific ligands to the surfaces of nanoparticles is important for medical and biologic imaging. However, covalent modification of nanoparticles has inherent problems in reproducibility because of many factors such as temperature, pH, concentration, and reaction time. Thus, we developed a method for modifying nanoparticles by encapsulation with specific ligand-conjugated amphiphiles. Methods: We synthesized special amphiphiles with a hydrophilic head and a long single-alkyl chain, such as arginine-glycine-aspartic acid-C18, mannose-C18, lactose-C18, and 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid-C18. And then we produced stable quantum dots (QDs) encapsulated with polysorbate 60 (a branched polyethylene glycol head with a C18 tail) and the synthesized special amphiphiles. The prepared encapsulated QDs were subject to in vitro and in vivo animal biodistribution studies and small-animal PET studies to confirm their specific binding. Results: The encapsulated QDs could specifically bind to target cells in vitro and in vivo and could be labeled with 68Ga (a positron emitter) easily and with high efficiency. Conclusion: The nanoparticles encapsulated with special amphiphiles could provide a straightforward and novel imaging solution with multimodality and multispecificity.