RT Journal Article SR Electronic T1 Investigating Vulnerable Atheroma Using Combined 18F-FDG PET/CT Angiography of Carotid Plaque with Immunohistochemical Validation JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 1698 OP 1703 DO 10.2967/jnumed.111.093724 VO 52 IS 11 A1 Menezes, Leon J. A1 Kotze, Carl W. A1 Agu, Obi A1 Richards, Toby A1 Brookes, Jocelyn A1 Goh, Vicky J. A1 Rodriguez-Justo, Manuel A1 Endozo, Raymondo A1 Harvey, Richard A1 Yusuf, Syed W. A1 Ell, Peter J. A1 Groves, Ashley M. YR 2011 UL http://jnm.snmjournals.org/content/52/11/1698.abstract AB Inflammation and angiogenesis are hypothesized to be important factors contributing to plaque vulnerability, whereas calcification is suggested to confer stability. To investigate this in vivo, we combined CT angiography and PET and compared the findings with immunohistochemistry for patients undergoing carotid endarterectomy. Methods: Twenty-one consecutive patients (18 men, 3 women; mean age ± SD, 68.3 ± 7.3) undergoing carotid endarterectomy were recruited for combined carotid 18F-FDG PET/CT angiography. Plaque 18F-FDG uptake was quantified with maximum standardized uptake value, and CT angiography quantified percentage plaque composition (calcium and lipid). Surgical specimens underwent ex vivo CT aiding image registration, followed by immunohistochemical staining for CD68 (macrophage density) and vascular endothelial growth factor (angiogenesis). Relationships between imaging and immunohistochemistry were assessed with Spearman rank correlation and multivariable regression. Results: The mean (±SD) surgically excised carotid plaque 18F-FDG metabolism was 2.4 (±0.5) versus 2.2 (±0.3) contralaterally (P = 0.027). There were positive correlations between plaque 18F-FDG metabolism and immunohistochemistry with CD68 (ρ = 0.55; P = 0.011) and vascular endothelial growth factor (ρ = 0.47; P = 0.031). There was an inverse relationship between plaque 18F-FDG metabolism and plaque percentage calcium composition on CT (ρ = −0.51; P = 0.018) and between calcium composition and immunohistochemistry with CD68 (ρ = −0.57; P = 0.007). Regression showed that maximum standardized uptake value and calcium composition were independently significant predictors of angiogenesis, and calcium composition was a predictor of macrophage density. Conclusion: We provide in vivo evidence that increased plaque metabolism is associated with increased biomarkers of angiogenesis and inflammation, whereas plaque calcification is inversely related to PET and histologic biomarkers of inflammation.