RT Journal Article
SR Electronic
T1 <sup>89</sup>Zr-Nanocolloidal Albumin–Based PET/CT Lymphoscintigraphy for Sentinel Node Detection in Head and Neck Cancer: Preclinical Results
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1580
OP 1584
DO 10.2967/jnumed.111.089557
VO 52
IS 10
A1 Heuveling, Derrek A.
A1 Visser, Gerard W.M.
A1 Baclayon, Marian
A1 Roos, Wouter H.
A1 Wuite, Gijs J.L.
A1 Hoekstra, Otto S.
A1 Leemans, C. René
A1 de Bree, Remco
A1 van Dongen, Guus A.M.S.
YR 2011
UL http://jnm.snmjournals.org/content/52/10/1580.abstract
AB Identifying sentinel nodes near the primary tumor remains a problem in, for example, head and neck cancer because of the limited resolution of current lymphoscintigraphic imaging when using 99mTc-nanocolloidal albumin. This study describes the development and evaluation of a nanocolloidal albumin–based tracer specifically dedicated for high-resolution PET detection. Methods: 89Zr was coupled to nanocolloidal albumin via the bifunctional chelate p-isothiocyanatobenzyldesferrioxamine B. Quality control tests, including particle size measurements, and in vivo biodistribution and imaging experiments in a rabbit lymphogenic metastasis model were performed. Results: Coupling of 89Zr to nanocolloidal albumin appeared to be efficient, resulting in a stable product with a radiochemical purity greater than 95%, without affecting the particle size. PET showed distinguished uptake of 89Zr-nanocolloidal albumin in the sentinel nodes, with visualization of lymphatic vessels, and with a biodistribution comparable to 99mTc-nanocolloidal albumin. Conclusion: 89Zr-nanocolloidal albumin is a promising tracer for sentinel node detection by PET.