PT  - JOURNAL ARTICLE
AU  - Marleen Melis
AU  - Erik Vegt
AU  - Mark W. Konijnenberg
AU  - Monique de Visser
AU  - Magda Bijster
AU  - Marcel Vermeij
AU  - Eric P. Krenning
AU  - Otto C. Boerman
AU  - Marion de Jong
TI  - Nephrotoxicity in Mice After Repeated Imaging Using <sup>111</sup>In-Labeled Peptides
AID  - 10.2967/jnumed.109.074310
DP  - 2010 Jun 01
TA  - Journal of Nuclear Medicine
PG  - 973--977
VI  - 51
IP  - 6
4099  - http://jnm.snmjournals.org/content/51/6/973.short
4100  - http://jnm.snmjournals.org/content/51/6/973.full
SO  - J Nucl Med2010 Jun 01; 51
AB  - We determined the renal radiation dose of a series of 111In-labeled peptides using animal SPECT. Because the animals' health deteriorated, renal toxicity was assessed. Methods: Wild-type and megalin-deficient mice were imaged repeatedly at 3- to 6-wk intervals to quantify renal retention after injection of 40–50 MBq of 111In-diethylenetriaminepentaacetic acid–labeled peptides (octreotide, exendin, octreotate, neurotensin, and minigastrin analogs), and the absorbed kidney radiation doses were estimated. Body weight, renal function parameters, and renal histology were determined at 16–20 wk after the first scan and compared with those in naive animals. Results: Because of high renal retention, 111In-diethylenetriaminepentaacetic acid–exendin-4 scans resulted in a 70-Gy kidney radiation dose in wild-type mice. Megalin-deficient kidneys received 20–40 Gy. The other peptides resulted in much lower renal doses. Kidney function monitoring indicated renal damage in imaged animals. Conclusion: Micro-SPECT enables longitudinal studies in 1 animal. However, long-term nephrotoxic effects may be induced after high renal radiation doses, even with 111In-labeled radiotracers.