RT Journal Article
SR Electronic
T1 Comparison of Initial Cell Retention and Clearance Kinetics After Subendocardial or Subepicardial Injections of Endothelial Progenitor Cells in a Canine Myocardial Infarction Model
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 413
OP 417
DO 10.2967/jnumed.109.069732
VO 51
IS 3
A1 Andrea J. Mitchell
A1 Eric Sabondjian
A1 Jane Sykes
A1 Lela Deans
A1 Wendy Zhu
A1 Xiangru Lu
A1 Qingping Feng
A1 Frank S. Prato
A1 Gerald Wisenberg
YR 2010
UL http://jnm.snmjournals.org/content/51/3/413.abstract
AB Neither intravenous nor intracoronary routes provide targeted stem cell delivery to recently infarcted myocardium in sufficient quantities. Direct routes appear preferable. However, most prior studies have used epicardial injections, which are not practical for routine clinical use. The objective of this study was to compare cell retention and clearance kinetics between a subepicardial and a subendocardial technique. Methods: We evaluated 7 dogs with each technique, using 111In-tropolone–labeled endothelial progenitor cells and serial SPECT/CT for 15 d after injection. Results: In vivo indium imaging demonstrated comparable degrees of retention: 57% ± 15% for the subepicardial injections and 54% ± 26% for the subendocardial injections. Clearance half-lives were also similar at 69 ± 26 and 60 ± 21 h, respectively. Conclusion: This study demonstrates that subendocardial injections, clinically more practical, show clearance kinetics comparable to those of subepicardial injections and will facilitate the ultimate clinical use of this treatment modality.