RT Journal Article
SR Electronic
T1 In-Line Radiolabeling: A Novel Continuous-Flow System for Commercial-Scale Protein Labeling
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1178
OP 1186
DO 10.2967/jnumed.108.056812
VO 50
IS 7
A1 Harris, Debra A.
A1 Pellikka, Raimo
A1 Gasser, Olga
A1 Blaeuenstein, Peter
A1 Waibel, Robert
A1 Schubiger, P. August
A1 King, Steven W.
A1 Parseghian, Missag H.
YR 2009
UL http://jnm.snmjournals.org/content/50/7/1178.abstract
AB A key limitation in developing radiotherapeutic proteins is the expense of manufacturing the drug in small batches using traditional reaction vessels. Removing limitations on the quantity of protein labeled at any one time significantly decreases the cost of production, and nowhere is the need for cost-effective radiotherapeutics more acute than in the treatment of cancer. Methods: We describe a novel method that can rapidly radiolabel, theoretically, unlimited amounts of protein, without causing significant damage to binding potency or structural integrity. Our process controls the reaction rate for the isotope and reactants as they simultaneously flow through a reaction tube. Results: We have demonstrated proof of principle by labeling nearly a gram of antibody with 481 GBq (13 Ci) of 131I during a single 30-min reaction run. Conclusion: Simple to construct, our system is already used to manufacture a radiolabeled antibody, both in the United States and in India, as part of clinical trials to treat glioblastoma multiforme. Modified, this system may be also applicable for nonradioactive labeling.