TY - JOUR T1 - Uptake of <sup>18</sup>F-Labeled 6-Fluoro-6-Deoxy-<span class="sc">d</span>-Glucose by Skeletal Muscle Is Responsive to Insulin Stimulation JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 912 LP - 919 DO - 10.2967/jnumed.109.062687 VL - 50 IS - 6 AU - Chandra Spring-Robinson AU - Visvanathan Chandramouli AU - William C. Schumann AU - Peter F. Faulhaber AU - Yanming Wang AU - Chunying Wu AU - Faramarz Ismail-Beigi AU - Raymond F. Muzic, Jr. Y1 - 2009/06/01 UR - http://jnm.snmjournals.org/content/50/6/912.abstract N2 - We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and 18F-labeled 6-fluoro-6-deoxy-d-glucose (18F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of 18F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport. Methods: Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia. Results: The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of 18F-6FDG was negligible in several tissues. Conclusion: 18F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer 18F-6FDG is sensitive to insulin stimulation. ER -