TY - JOUR T1 - Comparison of <sup>99m</sup>Tc- and <sup>18</sup>F-Ubiquicidin Autoradiography to Anti–<em>Staphylococcus aureus</em> Immunofluorescence in Rat Muscle Abscesses JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 995 LP - 999 DO - 10.2967/jnumed.108.050880 VL - 49 IS - 6 AU - Dagmar Salber AU - Johannes Gunawan AU - Karl-Josef Langen AU - Eva Fricke AU - Peter Klauth AU - Wolfgang Burchert AU - Sijtse Zijlstra Y1 - 2008/06/01 UR - http://jnm.snmjournals.org/content/49/6/995.abstract N2 - 99mTc-ubiquicidin (UBI) 29-41 is under clinical evaluation for discrimination between bacterial infection and unspecific inflammation. We compared the distribution of 99mTc-UBI 29-41, the potential PET tracers 18F-UBI 29-41 and 18F-UBI 28-41, and 3H-deoxyglucose (DG) in rat muscle abscesses to that of anti–Staphylococcus aureus immunofluorescent imaging. Methods: Calf abscesses were induced in 15 CDF-Fischer rats after inoculation of Staphylococcus aureus. One to 6 d later, either 18F-UBI 29-41 and 3H-DG (n = 5) or 18F-UBI 28-41 and 3H-DG (n = 6) or 99mTc-UBI 29-41 and 3H-DG (n = 4) were injected simultaneously. Dual-tracer autoradiography of the abscess area was compared with the distribution of bacteria and macrophages. Results: The UBI derivates exhibited increased uptake in the abscess area that partly matched 3H-DG uptake and macrophage infiltration but showed no congruity with areas that were highly positive for bacteria. Conclusion: A specific binding of UBI derivatives to Staphylococcus aureus in vivo could not be confirmed in this study. ER -