PT  - JOURNAL ARTICLE
AU  - Dagmar Salber
AU  - Johannes Gunawan
AU  - Karl-Josef Langen
AU  - Eva Fricke
AU  - Peter Klauth
AU  - Wolfgang Burchert
AU  - Sijtse Zijlstra
TI  - Comparison of <sup>99m</sup>Tc- and <sup>18</sup>F-Ubiquicidin Autoradiography to Anti–<em>Staphylococcus aureus</em> Immunofluorescence in Rat Muscle Abscesses
AID  - 10.2967/jnumed.108.050880
DP  - 2008 Jun 01
TA  - Journal of Nuclear Medicine
PG  - 995--999
VI  - 49
IP  - 6
4099  - http://jnm.snmjournals.org/content/49/6/995.short
4100  - http://jnm.snmjournals.org/content/49/6/995.full
SO  - J Nucl Med2008 Jun 01; 49
AB  - 99mTc-ubiquicidin (UBI) 29-41 is under clinical evaluation for discrimination between bacterial infection and unspecific inflammation. We compared the distribution of 99mTc-UBI 29-41, the potential PET tracers 18F-UBI 29-41 and 18F-UBI 28-41, and 3H-deoxyglucose (DG) in rat muscle abscesses to that of anti–Staphylococcus aureus immunofluorescent imaging. Methods: Calf abscesses were induced in 15 CDF-Fischer rats after inoculation of Staphylococcus aureus. One to 6 d later, either 18F-UBI 29-41 and 3H-DG (n = 5) or 18F-UBI 28-41 and 3H-DG (n = 6) or 99mTc-UBI 29-41 and 3H-DG (n = 4) were injected simultaneously. Dual-tracer autoradiography of the abscess area was compared with the distribution of bacteria and macrophages. Results: The UBI derivates exhibited increased uptake in the abscess area that partly matched 3H-DG uptake and macrophage infiltration but showed no congruity with areas that were highly positive for bacteria. Conclusion: A specific binding of UBI derivatives to Staphylococcus aureus in vivo could not be confirmed in this study.