RT Journal Article
SR Electronic
T1 Accurate Noninvasive Measurement of Infarct Size in Mice with High-Resolution PET
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1837
OP 1844
VO 47
IS 11
A1 Stegger, Lars
A1 Hoffmeier, Anne-Nadine
A1 Schäfers, Klaus P.
A1 Hermann, Sven
A1 Schober, Otmar
A1 Schäfers, Michael A.
A1 Theilmeier, Gregor
YR 2006
UL http://jnm.snmjournals.org/content/47/11/1837.abstract
AB Reliable, repeatable, and time-efficient noninvasive measurement of infarct size in mice with PET would benefit studies aimed at the exploration of biochemical and functional changes associated with acute myocardial infarction (MI). PET with the radioactively labeled glucose derivative 18F-FDG is used in humans to distinguish between viable but dysfunctional and nonviable myocardium. In this study, the feasibility, accuracy, and time efficiency of 18F-FDG PET for quantification of infarct size in mice using a high-resolution animal PET device was evaluated in comparison with histomorphometry. Methods: Mice were subjected to surgery with permanent ligation of the left anterior descending artery. PET was performed before and 7 d after surgery. The infarct size was determined from the PET studies using both manual and automated delineation. The second PET scan was followed by histomorphometric analysis. Results: An excellent correlation between PET and histomorphometry was found for both manual (R = 0.98) and automated (R = 0.98) delineation, with linear regression curves close to unity (manual: y = 1.10x − 0.01; automated: y = 1.12x − 0.02). Automated analysis required <1 min per study. Conclusion: The measurement of infarct size in mice with 18F-FDG PET is feasible and highly accurate. This noninvasive methodology permits unique longitudinal studies of biochemical parameters in mice and facilitates studies that aim to assess the effect of surgical and pharmacologic intervention after acute MI.