PT  - JOURNAL ARTICLE
AU  - Igarashi, Norio
AU  - Nozawa, Takashi
AU  - Fujii, Nozomu
AU  - Kato, Bun-ichi
AU  - Nonomura, Makoto
AU  - Matsuki, Akira
AU  - Nakadate, Teruo
AU  - Igawa, Akihiko
AU  - Asanoi, Hidetsugu
AU  - Inoue, Minoru
AU  - Inoue, Hiroshi
TI  - Evaluation of Fatty Acid Metabolism in Hearts After Ischemia–Reperfusion Injury Using a Dual-Isotope Autoradiographic Approach and Tissue Assay for Metabolites of Tracer
DP  - 2005 Jan 01
TA  - Journal of Nuclear Medicine
PG  - 160--164
VI  - 46
IP  - 1
4099  - http://jnm.snmjournals.org/content/46/1/160.short
4100  - http://jnm.snmjournals.org/content/46/1/160.full
SO  - J Nucl Med2005 Jan 01; 46
AB  - We investigated whether changes in myocardial uptake of fatty acid tracer after reperfusion following transient myocardial ischemia were closely related to alterations in intracellular fatty acid oxidation. Methods: Using a fatty acid tracer of 131I- and 125I-labeled 15-(p-iodophenyl)-9-methylpentadecanoic acid (9MPA), the myocardial uptake and metabolites were determined by dual-tracer autoradiography and thin-layer chromatography in rats 3 or 14 d after reperfusion following 5 or 15 min of ischemia induced by coronary artery ligation. Results: 9MPA metabolites processed via β-oxidation were lower in the ischemic region (IR) than in non-IR 3 d after 5 min of ischemia, despite no reduction of tracer uptake in IR. Oxidation of 9MPA was recovered 14 d after 15 min of ischemia in association with normalization of tracer uptake in IR, whereas both uptake and oxidation of 9MPA were markedly impaired 3 d after 15 min of ischemia, accompanied by slow clearance of myocardial tracer. Conclusion: Normal uptake of fatty acid tracer early after reperfusion does not always imply preserved intracellular fatty acid oxidation. However, reduction of tracer uptake might reflect impaired fatty acid oxidation.