TY - JOUR T1 - Accuracy of a Method Using Short Inhalation of <sup>15</sup>O-O<sub>2</sub> for Measuring Cerebral Oxygen Extraction Fraction with PET in Healthy Humans JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 765 LP - 770 VL - 45 IS - 5 AU - Naoya Hattori AU - Marvin Bergsneider AU - Hsiao-Ming Wu AU - Thomas C. Glenn AU - Paul M. Vespa AU - David A. Hovda AU - Michael E. Phelps AU - Sung-Cheng Huang Y1 - 2004/05/01 UR - http://jnm.snmjournals.org/content/45/5/765.abstract N2 - PET with short inhalation of 15O-O2 provides regional oxygen extraction fraction (OEF) in a shorter acquisition time and with less radiation exposure than does the steady-state method. The purpose of this study was to test the accuracy of the short-inhalation technique for estimating OEF in healthy human volunteers. Methods: The final study population included 16 healthy volunteers, who underwent a series of dynamic PET scans consisting of short inhalation of 15O-CO, short inhalation of 15O-O2, and a bolus infusion of 15O-H2O to generate parametric images for cerebral blood volume (CBV), cerebral blood flow (CBF), OEF, and metabolic rate of oxygen (CMRO2). About 45 min before PET emission scanning, arterial and jugular blood was sampled through a catheter inserted in a radial artery and the right jugular bulb, respectively. PET-derived OEF (OEFpet) of the whole brain was compared with OEF calculated from the arteriovenous blood-sampling technique (OEFav). Results: Whole-brain-averaged CBF (mean ± SD) measured with PET was 0.40 ± 0.06 (range, 0.30–0.55) mL/g/min, CBV was 0.05 ± 0.01 (range, 0.04–0.09) mL/g, CMRO2 was 2.85 ± 0.39 (range, 2.35–3.84) mL/100 g/min, and OEFpet was 0.39 ± 0.06 (range, 0.30–0.51). OEFpet showed a slightly higher value than did OEFav (0.36 ± 0.05 [range, 0.29–0.46]), but the difference was not significant. The difference in the 2 measurements (OEFpet - OEFav) did not correlate with CBF (r = −0.16; P = not statistically significant [NS]), CBV (r = −0.20; P = NS), CMRO2 (r = −0.16; P = NS), partial arterial oxygen pressure (r = 0.29; P = NS) or partial arterial carbon dioxide pressure (r = −0.17; P = NS). Conclusion: Compared with the arteriovenous blood-sampling technique, a technique using short inhalation of 15O-O2 did not significantly over- or underestimate global OEF in healthy human volunteers. The PET technique reasonably estimated the cerebral OEF in local brain tissues of healthy human volunteers. ER -