RT Journal Article
SR Electronic
T1 Multimodality Noninvasive Imaging of Gene Transfer Using the Human Sodium Iodide Symporter
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 445
OP 449
VO 45
IS 3
A1 Niu, Gang
A1 Gaut, Andrew W.
A1 Ponto, Laura L. Boles
A1 Hichwa, Richard D.
A1 Madsen, Mark T.
A1 Graham, Michael M.
A1 Domann, Frederick E.
YR 2004
UL http://jnm.snmjournals.org/content/45/3/445.abstract
AB In this study we investigated the feasibility of using radionuclide accumulation mediated by the human sodium iodide symporter (hNIS) gene in conjunction with various imaging modalities as a reporter system to noninvasively monitor the expression of transgenes delivered for gene therapy. Methods: NIS-expressing adenovirus (Ad-hNIS) was delivered in vitro to MB-435 breast carcinoma cells. NIS-mediated accumulation of 125I−, 99mTcO4−, and 76Br− by the cells was visualized using autoradiography, γ-camera scintigraphy, and PET imaging, respectively. Results: For all imaging modalities, signal intensity generated by the cells correlated linearly both with the amount of Ad-hNIS and with the activity of radionuclide added to the cells. Conclusion: hNIS-mediated cellular accumulation of radionuclide was clearly visualized by all 3 imaging modalities tested. This preliminary study demonstrates the feasibility of using hNIS for monitoring the location and magnitude of expression of genes delivered during gene therapy.