RT Journal Article
SR Electronic
T1 Quantitative Analysis of (−)-<em>N</em>-<sup>11</sup>C-Propyl-Norapomorphine In Vivo Binding in Nonhuman Primates
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 338
OP 346
VO 45
IS 2
A1 Dah-Ren Hwang
A1 Rajesh Narendran
A1 Yiyun Huang
A1 Mark Slifstein
A1 Peter S. Talbot
A1 Yasuhiko Sudo
A1 Bart N. Van Berckel
A1 Lawrence S. Kegeles
A1 Diana Martinez
A1 Marc Laruelle
YR 2004
UL http://jnm.snmjournals.org/content/45/2/338.abstract
AB (−)-N-11C-propyl-norapomorphine (11C-NPA) is a new dopamine agonist PET radiotracer that holds potential for imaging the high-affinity states of dopamine D2-like receptors in the living brain. The goal of this study was to develop and evaluate analytic strategies to derive in vivo 11C-NPA binding parameters. Methods: Two baboons were scanned 4 times after 11C-NPA injections. The metabolite-corrected arterial input functions were measured. Regional brain time-activity curves were analyzed with kinetic and graphical analyses, using the arterial time-activity curve as the input function. Data were also analyzed with the simplified reference-tissue model (SRTM) and graphical analysis with reference-region input. Results: 11C-NPA exhibited moderately fast metabolism, with 31% ± 5% of arterial plasma concentration corresponding to the parent compound at 40 min after injection. Plasma clearance was 29 ± 1 L/h, and plasma free fraction (f1) was 5% ± 1%. For kinetic analysis, a 1-tissue compartment model (1TCM) provided a good fit to the data and more robust derivations of the tissue distribution volumes (VT, in mL/g) than a 2-tissue compartment model (2TCM). Using 1TCM, VTs in the cerebellum and striatum were 3.4 ± 0.4 and 7.5 ± 2 mL/g, respectively, which led to estimates of striatal binding potential (BP) of 4.0 ± 1.1 mL/g and striatal equilibrium specific-to-nonspecific partition coefficient (V3″) of 1.2 ± 0.2. VT values derived with graphical analysis were well correlated with but slightly lower than VT values derived with kinetic analysis. V3″ values derived with SRTM were well correlated with but slightly higher than V3″ values derived with kinetic analysis. Using any method, a significant difference was detected in BP and V3″ values between the 2 animals. It was determined that 30 min of scanning data were sufficient to derive V3″ values using kinetic, graphical (arterial input and reference-region input), and SRTM analyses. Conclusion: This study indicates that 11C-NPA is a suitable PET tracer to quantify the agonist high-affinity sites of D2-like receptors.