PT  - JOURNAL ARTICLE
AU  - Yasuhiro Magata
AU  - Takahiro Mukai
AU  - Masafumi Ihara
AU  - Sadahiko Nishizawa
AU  - Haruhiro Kitano
AU  - Koichi Ishizu
AU  - Hideo Saji
AU  - Junji Konishi
TI  - Simple Analytic Method of <sup>11</sup>C-Flumazenil Metabolite in Blood
DP  - 2003 Mar 01
TA  - Journal of Nuclear Medicine
PG  - 417--421
VI  - 44
IP  - 3
4099  - http://jnm.snmjournals.org/content/44/3/417.short
4100  - http://jnm.snmjournals.org/content/44/3/417.full
SO  - J Nucl Med2003 Mar 01; 44
AB  - 11C-Flumazenil (11C-FMZ) is useful to estimate central benzodiazepine receptors by PET. The binding potential (BP) can be calculated with dynamic PET and continual blood sampling. Because conventional metabolite analysis of plasma samples is complicated and time consuming, a simple method is required to obtain an input function. In this article, a whole blood solvent extraction method was evaluated using data of 13 subjects. Methods: The plasma solvent extraction method was estimated in comparison with the thin-layer chromatography (TLC) method. Then, the whole blood solvent extraction method was evaluated in comparison with the plasma solvent extraction method. Results: Metabolite data analyzed by the plasma extraction method were well correlated with those by the TLC method (r = 0.99). The BP was calculated using both the whole blood extraction data and the plasma extraction data. No difference was observed in all brain regions. Conclusion: This simple whole blood solvent extraction method can be applied to clinical BP estimation using 11C-FMZ.