PT - JOURNAL ARTICLE AU - Lorberboym, Mordechai AU - Lampl, Yair AU - Sadeh, Menahem TI - Correlation of <sup>99m</sup>Tc-DTPA SPECT of the Blood–Brain Barrier with Neurologic Outcome After Acute Stroke DP - 2003 Dec 01 TA - Journal of Nuclear Medicine PG - 1898--1904 VI - 44 IP - 12 4099 - http://jnm.snmjournals.org/content/44/12/1898.short 4100 - http://jnm.snmjournals.org/content/44/12/1898.full SO - J Nucl Med2003 Dec 01; 44 AB - We conducted a study on humans to determine whether quantitative evaluation of blood–brain barrier (BBB) breakdown using the 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA) SPECT technique at the peak time of stroke evolution can predict neurologic outcome. Methods: Thirty consecutive patients with acute stroke of the middle cerebral artery occurring &gt;24 h and &lt;48 h before admission were included in the study. Each patient underwent a complete neurologic examination according to the Scandinavian stroke score at 72 h after the stroke (S1) and again at 30 d (S2). The difference between initial and late scores was calculated (Δ S) and used to evaluate the change in neurologic status. A CT scan was obtained on all patients to determine the volume of stroke. The integrity of the BBB was evaluated using 99mTc-DTPA brain SPECT. A quantitative index of BBB disruption was defined as the ratio of the mean counts/pixel in the infarcted region compared with the mean counts/pixel in the contralateral nonaffected hemisphere. SPECT perfusion imaging was also performed with 99mTc-hexamethylpropyleneamine oxime (99mTc-HMPAO) at 24 h after the 99mTc-DTPA study. The relative perfusion in the infarct region was expressed as the percentage of contralateral perfusion. Results: The mean 99mTc-DTPA disruption index was 6.8 ± 6.9 (range, 1–26.2). Seven patients (23%) had no BBB disruption. Statistical analysis showed that the disruption index was negatively correlated with Δ S (r = −0.423, P &lt; 0.02). A disruption index of &lt;2.5 was associated with a significantly better neurologic outcome (mean Δ S, 17.5 ± 9.5) compared with patients with an index of &gt;2.5 (mean Δ S, −0.85 ± 4.97, P &lt; 0.0001) with a sensitivity of 95% and a specificity of 89%. S2 was significantly correlated with S1 (r = 0.738, P &lt; 0.001) and with Δ S (r = 0.656, P &lt; 0.001). Perfusion abnormalities on the 99mTc-HMPAO SPECT studies ranged between 12% and 90% (mean, 37.6% ± 17.8%) compared with those on the contralateral nonaffected side. No correlation was found between 99mTc-HMPAO uptake and Δ S, infarct volume by CT, or disruption index. The CT volume measurements were negatively correlated with S2 (r = −0.560, P &lt; 0.004) but not with Δ S. Conclusion: 99mTc-DTPA SPECT of the BBB combined with quantitative analysis in patients with acute stroke is significantly related to clinical outcome, with a distinct prognostic cutoff threshold of 2.5. The use of this radionuclide brain SPECT technique represents a unique application of conventional nondiffusible brain agents.