@article {M{\'e}ndez1677, author = {Jeanette M{\'e}ndez and Anne M. Wallace and Carl K. Hoh and David R. Vera}, title = {Detection of Gastric and Colonic Sentinel Nodes Through Endoscopic Administration of 99mTc-DTPA-Mannosyl-Dextran in Pigs }, volume = {44}, number = {10}, pages = {1677--1681}, year = {2003}, publisher = {Society of Nuclear Medicine}, abstract = {The purpose of this study was to develop a method for the endoscopic administration of a radiopharmaceutical for sentinel node detection and to characterize its uptake by gastric and colonic sentinel nodes. 99mTc-Diethylenetriaminepentaacetic acid (DTPA)-mannosyl-dextran is a new radiotracer labeled with 99mTc and composed of multiple units of mannose attached to the polymeric backbone, dextran. Methods: Gastric and colonic lymph node detection was studied in 4 fasting and anesthetized pigs. A flexible video endoscope was inserted into the rectum and positioned in the lower colon or advanced down the esophagus into the stomach. A standard endoscopic sclerotherapy needle and sheath filled with 0.9\% saline was then loaded with 0.2 mL of a 7.4-MBq 1:1 (v/v) mixture of isosulfan blue and 99mTc-DTPA-mannosyl-dextran (7.4 MBq, 0.3 nmol). The needle and sheath were passed through the biopsy channel, and the radiotracer/dye solution (0.1 mL) was injected in a tangential fashion into the submucosa. Within 5{\textendash}10 min after injection, all radioactive or blue lymph nodes were excised, assayed for radioactivity, and noted for color. Distal lymph nodes were also excised, assayed, and noted for color. Results: 99mTc-DTPA-mannosyl-dextran uptake by colonic sentinel nodes (n = 4) ranged from 0.54\% to 2.4\% of the injected dose; all radioactive nodes were stained blue. The uptake for all (n = 3) distal nodes ranged from 0.001\% to 0.005\%, and none of these nodes were stained blue. Uptake by gastric sentinel nodes (n = 6) ranged from 0.13\% to 4.50\%; all radioactive nodes were stained blue. The range for distal nodes was 0.001\% to 0.050\%; no distal nodes were stained blue. In 2 pigs, each gastric injection produced 2 hot and blue lymph nodes. Conclusion: Within 10 min of a gastric and colonic submucosal injection, 99mTc-DTPA-mannosyl-dextran demonstrated high sentinel node uptake and high concordance with isosulfan blue.}, issn = {0161-5505}, URL = {https://jnm.snmjournals.org/content/44/10/1677}, eprint = {https://jnm.snmjournals.org/content/44/10/1677.full.pdf}, journal = {Journal of Nuclear Medicine} }