RT Journal Article
SR Electronic
T1 Rat Studies Comparing <sup>11</sup>C-FMAU, <sup>18</sup>F-FLT, and <sup>76</sup>Br-BFU as Proliferation Markers
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1688
OP 1698
VO 43
IS 12
A1 Li Lu
A1 Linda Samuelsson
A1 Mats Bergström
A1 Kengo Sato
A1 Karl-Johan Fasth
A1 Bengt Långström
YR 2002
UL http://jnm.snmjournals.org/content/43/12/1688.abstract
AB We analyzed and compared 1-(2′-deoxy-2′-fluoro-β-d-arabinofuranosyl)-[methyl-11C]thymine (11C-FMAU), 3′-deoxy-3′-[18F]fluorothymidine (18F-FLT) and 1-(2′-deoxy-2′-fluoro-β-d-arabinofuranosyl)-5-[76Br]bromouracil (76Br-BFU) with respect to tissue uptake, DNA incorporation, and excretion modulation in rats. The goal of the investigation was to evaluate the efficiency of the 3 nucleoside tracers as potential tracers for measuring proliferation. Methods: Sprague-Dawley rats were divided into 3 groups and administered 5 MBq 11C-FMAU, 1 MBq 18F-FLT, or 2 MBq 76Br-BFU. For each tracer, a subgroup was also administered 6 mg/kg cimetidine. The rats in the 11C-FMAU group were killed at 5, 20, 40, 60, and 80 min after injection; the rats in the 18F-FLT group were killed at 80 min and 2 and 4 h; and the rats in the 76Br-BFU group were killed at 5, 20, 40, and 80 min and 2, 4, 6, and 24 h. Samples of blood, liver, kidney, spleen, and intestine were taken, and the radioactivity was measured. DNA separation was made in the samples of spleen, and the radioactivity in the DNA fraction was measured. Results: Maximal uptake of radioactivity was seen in the spleen and intestine, organs with active DNA synthesis. The highest relative radioactivity uptake was at 60 min in the 11C-FMAU groups and at 4 h in the 18F-FLT group. In the 76Br-BFU group, the uptake increased gradually during the observation period, and uptake of radioactivity increased markedly in rats receiving cimetidine. Cimetidine did not affect radioactivity uptake in the 11C-FMAU or 18F-FLT groups. The fraction of radioactivity in DNA was 78% in spleen at 60 min in the 11C-FMAU group, 80% at 60 min and 97% at 4 h in the 76Br-BFU group. The DNA-incorporation was only 2% in the 18F-FLT group. Conclusion: 76Br-BFU predominantly incorporates into DNA and has great potential as a PET tracer for the assessment of proliferation in vivo. 11C-FMAU also may have potential as a proliferation marker, but the observation time is limited. 18F-FLT does not incorporate into DNA and is therefore not a direct marker of proliferation.