PT  - JOURNAL ARTICLE
AU  - Passchier, Jan
AU  - van Waarde, Aren
AU  - Vaalburg, Willem
AU  - Willemsen, Antoon T.M.
TI  - On the Quantification of [<sup>18</sup>F]MPPF Binding to 5-HT<sub>1A</sub> Receptors in the Human Brain
DP  - 2001 Jul 01
TA  - Journal of Nuclear Medicine
PG  - 1025--1031
VI  - 42
IP  - 7
4099  - http://jnm.snmjournals.org/content/42/7/1025.short
4100  - http://jnm.snmjournals.org/content/42/7/1025.full
SO  - J Nucl Med2001 Jul 01; 42
AB  - Previous studies have shown that 4-(2′-methoxyphenyl)-1-[2′-(N-2″-pyridinyl)-p-[18F]fluorobenzamido]ethylpiperazine ([18F]MPPF) binds with high selectivity to serotonin (5-HT1A) receptors in man. However, in these studies, the calculation of the binding potential (BP, which equals receptor density divided by equilibrium dissociation constant) used a metabolite-corrected arterial input. The aim of this study was to determine whether metabolite correction and arterial sampling are essential for the assessment of BP. Methods: Five analytic methods using full datasets obtained from 6 healthy volunteers were compared. In addition, the clinical applicability of these methods was appraised. Three methods were based on Logan analysis of the dynamic PET data using metabolite-corrected and uncorrected arterial plasma input and cerebellar input. The other 2 methods consisted of a simplified reference tissue model and standard compartmental modeling. Results: A high correlation was found between BP calculated with Logan analysis using the metabolite-corrected plasma input (used as the reference method for this study) and Logan analysis using either the uncorrected arterial plasma input (r2 = 0.95, slope = 0.85) or cerebellar input (r2 = 0.98, slope = 0.91). A high correlation was also found between our reference method and the simplified reference tissue model (r2 = 0.94, slope = 0.92). In contrast, a poor correlation was observed between our reference method and the standard compartmental model (r2 = 0.45, slope = 1.59). Conclusion: These results indicate that neither metabolite analysis nor arterial sampling is necessary for clinical evaluation of BP in the human brain with [18F]MPPF. Both the Logan analysis method with cerebellar input and the simplified reference tissue method can be applied clinically.