PT  - JOURNAL ARTICLE
AU  - Jinshan Zhou
AU  - Kotaro Higashi
AU  - Yoshimichi Ueda
AU  - Yuko Kodama
AU  - Dachuan Guo
AU  - Fumiko Jisaki
AU  - Aya Sakurai
AU  - Tsutomu Takegami
AU  - Shogo Katsuda
AU  - Itaru Yamamoto
TI  - Expression of Multidrug Resistance Protein and Messenger RNA Correlate with <sup>99m</sup>Tc-MIBI Imaging in Patients with Lung Cancer
DP  - 2001 Oct 01
TA  - Journal of Nuclear Medicine
PG  - 1476--1483
VI  - 42
IP  - 10
4099  - http://jnm.snmjournals.org/content/42/10/1476.short
4100  - http://jnm.snmjournals.org/content/42/10/1476.full
SO  - J Nucl Med2001 Oct 01; 42
AB  - In vitro studies have shown that 99mTc-sestamibi (MIBI) is a transport substrate for the P-glycoprotein (Pgp) pump and the multidrug resistance–associated protein (MRP) pump. However, whether MRP and lung resistance protein (LRP) affect tumor accumulation and efflux of 99mTc-MIBI in lung cancer is not known. In this study, we explored whether Pgp and the other pumps, MRP and LRP, affect tumor accumulation and efflux of 99mTc-MIBI in lung cancer. Methods: Thirty-four lung cancer patients who underwent surgery were examined. Before surgery, 99mTc-MIBI SPECT was performed 15 min and 180 min after injection, and early uptake, delayed uptake (L/Nd), and washout rate (L/Nwr) of 99mTc-MIBI were obtained. Pgp, MRP, and LRP expression were investigated by immunohistochemistry. The messenger RNA (mRNA) level of Pgp, MRP, and LRP was determined by real-time reverse-transcription polymerase chain reaction. The lung cancer 99mTc-MIBI images were correlated with protein and mRNA expression. Results: The mean L/Nd of the Pgp (−) group was significantly higher than that of the Pgp (++) group (P = 0.0324). The Pgp (++) group had a higher L/Nwr than did the Pgp (−) group (P = 0.0269). The mean L/Nd of the Pgp mRNA low-expression group was significantly higher than that of the Pgp mRNA high-expression group (P = 0.0127). The Pgp mRNA high-expression group had a higher L/Nwr than did the Pgp mRNA low-expression group (P = 0.0825). No appreciable correlation was found between the lung cancer 99mTc-MIBI images and the expression of MRP or LRP on the level of protein or mRNA. Conclusion: These data suggest that an increased level of Pgp expression correlates with a low accumulation on delayed scans and a high L/Nwr of 99mTc-MIBI in lung cancer. Neither MRP nor LRP expression on the level of either protein or mRNA correlated significantly with tumor accumulation or efflux of 99mTc-MIBI in lung cancer.