PT  - JOURNAL ARTICLE
AU  - Chan, Chung Ying
AU  - Chen, Zijun
AU  - Guibbal, Florian
AU  - Dias, Gemma
AU  - Destro, Gianluca
AU  - O’Neill, Edward
AU  - Veal, Mathew
AU  - Lau, Doreen
AU  - Mosley, Michael
AU  - Wilson, Thomas C.
AU  - Gouverneur, Véronique
AU  - Cornelissen, Bart
TI  - [<sup>123</sup>I]CC1: A PARP-Targeting, Auger Electron–Emitting Radiopharmaceutical for Radionuclide Therapy of Cancer
AID  - 10.2967/jnumed.123.265429
DP  - 2023 Dec 01
TA  - Journal of Nuclear Medicine
PG  - 1965--1971
VI  - 64
IP  - 12
4099  - http://jnm.snmjournals.org/content/64/12/1965.short
4100  - http://jnm.snmjournals.org/content/64/12/1965.full
SO  - J Nucl Med2023 Dec 01; 64
AB  - Poly(adenosine diphosphate ribose) polymerase (PARP) has emerged as an effective therapeutic strategy against cancer that targets the DNA damage repair enzyme. PARP-targeting compounds radiolabeled with an Auger electron–emitting radionuclide can be trapped close to damaged DNA in tumor tissue, where high ionizing potential and short range lead Auger electrons to kill cancer cells through the creation of complex DNA damage, with minimal damage to surrounding normal tissue. Here, we report on [123I]CC1, an 123I-labeled PARP inhibitor for radioligand therapy of cancer. Methods: Copper-mediated 123I iododeboronation of a boronic pinacol ester precursor afforded [123I]CC1. The level and specificity of cell uptake and the therapeutic efficacy of [123I]CC1 were determined in human breast carcinoma, pancreatic adenocarcinoma, and glioblastoma cells. Tumor uptake and tumor growth inhibition of [123I]CC1 were assessed in mice bearing human cancer xenografts (MDA-MB-231, PSN1, and U87MG). Results: In vitro and in vivo studies showed selective uptake of [123I]CC1 in all models. Significantly reduced clonogenicity, a proxy for tumor growth inhibition by ionizing radiation in vivo, was observed in vitro after treatment with as little as 10 Bq [123I]CC1. Biodistribution at 1 h after intravenous administration showed PSN1 tumor xenograft uptake of 0.9 ± 0.06 percentage injected dose per gram of tissue. Intravenous administration of a relatively low amount of [123I]CC1 (3 MBq) was able to significantly inhibit PSN1 xenograft tumor growth but was less effective in xenografts that expressed less PARP. [123I]CC1 did not cause significant toxicity to normal tissues. Conclusion: Taken together, these results show the potential of [123I]CC1 as a radioligand therapy for PARP-expressing cancers.