RT Journal Article
SR Electronic
T1 PET Imaging of System x<sub>C</sub><sup>−</sup> in Immune Cells for Assessment of Disease Activity in Mice and Patients with Inflammatory Bowel Disease
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 1586
OP 1591
DO 10.2967/jnumed.121.263289
VO 63
IS 10
A1 Minjung Seo
A1 Yeji Kim
A1 Byong Duk Ye
A1 Sang Hyoung Park
A1 Seog-Young Kim
A1 Jin Hwa Jung
A1 Sung Wook Hwang
A1 Sun Young Chae
A1 Dong Yun Lee
A1 Sang Ju Lee
A1 Seung Jun Oh
A1 Jihun Kim
A1 Ji Young Kim
A1 Sae Jung Na
A1 Misung Kim
A1 Sang-Yeob Kim
A1 Norman Koglin
A1 Andrew W. Stephens
A1 Mi-Na Kweon
A1 Dae Hyuk Moon
YR 2022
UL http://jnm.snmjournals.org/content/63/10/1586.abstract
AB We aimed to explore whether the imaging of antiporter system xC− of immune cells with (4S)-4-(3-18F-fluoropropyl)-l-glutamate (18F-FSPG) PET can assess inflammatory bowel disease (IBD) activity in murine models and patients (NCT03546868). Methods: 18F-FSPG PET imaging was performed to assess IBD activity in mice with dextran sulfate sodium-induced and adoptive T-cell transfer–induced IBD and a cohort of 20 patients at a tertiary care center in South Korea. Immunohistochemical analysis of system xC− and cell surface markers was also studied. Results: Mice with experimental IBD showed increased intestinal 18F-FSPG uptake and xCT expression in cells positive (+) for CD11c, F4/80, and CD3 in the lamina propria, increases positively associated with clinical and pathologic disease activity. 18F-FSPG PET studies in patients, most of whom were clinically in remission or had mildly active IBD, showed that PET imaging was sufficiently accurate in diagnosing endoscopically active IBD and remission in patients and bowel segments. 18F-FSPG PET correctly identified all 9 patients with superficial or deep ulcers. Quantitative intestinal 18F-FSPG uptake was strongly associated with endoscopic indices of IBD activity. The number of CD68+xCT+ and CD3+xCT+ cells in 22 bowel segments from patients with ulcerative colitis and the number of CD68+xCT+ cells in 7 bowel segments from patients with Crohn disease showed a significant positive association with endoscopic indices of IBD activity. Conclusion: The assessment of system xC− in immune cells may provide diagnostic information on the immune responses responsible for chronic active inflammation in IBD. 18F-FSPG PET imaging of system xC− activity may noninvasively assess the IBD activity.