TY - JOUR T1 - PET Imaging of System x<sub>C</sub><sup>−</sup> in Immune Cells for Assessment of Disease Activity in Mice and Patients with Inflammatory Bowel Disease JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1586 LP - 1591 DO - 10.2967/jnumed.121.263289 VL - 63 IS - 10 AU - Minjung Seo AU - Yeji Kim AU - Byong Duk Ye AU - Sang Hyoung Park AU - Seog-Young Kim AU - Jin Hwa Jung AU - Sung Wook Hwang AU - Sun Young Chae AU - Dong Yun Lee AU - Sang Ju Lee AU - Seung Jun Oh AU - Jihun Kim AU - Ji Young Kim AU - Sae Jung Na AU - Misung Kim AU - Sang-Yeob Kim AU - Norman Koglin AU - Andrew W. Stephens AU - Mi-Na Kweon AU - Dae Hyuk Moon Y1 - 2022/10/01 UR - http://jnm.snmjournals.org/content/63/10/1586.abstract N2 - We aimed to explore whether the imaging of antiporter system xC− of immune cells with (4S)-4-(3-18F-fluoropropyl)-l-glutamate (18F-FSPG) PET can assess inflammatory bowel disease (IBD) activity in murine models and patients (NCT03546868). Methods: 18F-FSPG PET imaging was performed to assess IBD activity in mice with dextran sulfate sodium-induced and adoptive T-cell transfer–induced IBD and a cohort of 20 patients at a tertiary care center in South Korea. Immunohistochemical analysis of system xC− and cell surface markers was also studied. Results: Mice with experimental IBD showed increased intestinal 18F-FSPG uptake and xCT expression in cells positive (+) for CD11c, F4/80, and CD3 in the lamina propria, increases positively associated with clinical and pathologic disease activity. 18F-FSPG PET studies in patients, most of whom were clinically in remission or had mildly active IBD, showed that PET imaging was sufficiently accurate in diagnosing endoscopically active IBD and remission in patients and bowel segments. 18F-FSPG PET correctly identified all 9 patients with superficial or deep ulcers. Quantitative intestinal 18F-FSPG uptake was strongly associated with endoscopic indices of IBD activity. The number of CD68+xCT+ and CD3+xCT+ cells in 22 bowel segments from patients with ulcerative colitis and the number of CD68+xCT+ cells in 7 bowel segments from patients with Crohn disease showed a significant positive association with endoscopic indices of IBD activity. Conclusion: The assessment of system xC− in immune cells may provide diagnostic information on the immune responses responsible for chronic active inflammation in IBD. 18F-FSPG PET imaging of system xC− activity may noninvasively assess the IBD activity. ER -