TY - JOUR T1 - <strong>Fully automated, commercial cassette-based production of high-purity [64Cu]CuCl2 from solid Ni target</strong> JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 2754 LP - 2754 VL - 63 IS - supplement 2 AU - Courtney Hollis AU - Hiu Lam AU - William Tieu AU - Mario Malinconico Y1 - 2022/06/01 UR - http://jnm.snmjournals.org/content/63/supplement_2/2754.abstract N2 - 2754 Introduction: Copper-64 (64Cu) is an attractive radionuclide for nuclear medical imaging. This radionuclide decays with low energy positron emission (β+ 17.6%, Eavg = 653 keV) and intermediate half-life (t1/2 = 12.7 h). 64Cu is amenable for labelling both short (minutes) and long (hours/days) incubating biological vectors and is exemplified by the many reports of 64Cu labelled small molecule, peptides, proteins and nanoparticles. Clinical studies with 64Cu have also progressed significantly in the past two decades. This work focuses on obtaining high-purity [64Cu]CuCl2 via a cassette-based automated separation method using a one column approach implemented on the Taddeo-PRF chemistry platform. Methods: 64Ni is electroplated onto a target shuttle and irradiated with 14.4 MeV protons to produce 64Cu. The irradiated target is dissolved and purified to obtain [64Cu]CuCl2. Two purification methods are tested on the ALCEO system (COMECER); 1. AG 1-X8 resin (Biorad); 2. TK201 resin (Triskem). ICP-MS has been performed to monitor the quality of [64Cu]CuCl2.Results: 64Cu is produced using the AG 1-X8 method with a yield of 2.49 ± 0.67 MBq/µAh/mg (N=21), electroplating 53.93 ± 20.42 mg of 64Ni and bombarding at 35 ± 0.2 µA for 247.95 ± 74.74 minutes with a proton beam. For the TK201 method, the yield is 2.30 ± 0.83 MBq/µAh/mg (N=61), electroplating 69.50 ± 17.61 mg of 64Ni and bombarding at 35 ± 0.2 µA for 201.13 ± 58.10 minutes. ICP-MS shows for the AG 1-X8 method (N=12): [Ni-64] 70728 ± 81643 ppb, [Cu] 1125 ± 657 ppb, [Fe] 8040 ± 4287 ppb, [Al] 3303 ± 1925 ppb, [Zn] 2336 ± 2602 ppb, [Co] 33 ± 35 ppb. Instead, for the TK201 method shows (N=30): [Ni-64] 8966 ± 11045 ppb, [Cu] 1244 ± 1636 ppb, [Fe] 426 ± 408 ppb, [Al] 1499 ± 2042 ppb, [Zn] 2717 ± 7196 ppb, [Co] 66 ± 84 ppb. Conclusions: An automated method capable of producing high purity 64Cu from 64Ni targets has been developed. In summary, the entire process can be achieved in ~ 120 min (60 min for dissolution and resin loading, 30 min for resin elution and 30 min for evaporation of the 0.5 M acid)  min from EoB to EoS, with average yields of 2.54 ± 0.8 MBq/µAh/mg when irradiating with 35 μA for 2.5 to 3.5 h. The quality of the final product has been tested and it is suitable for all labelling processes. ER -