RT Journal Article SR Electronic T1 A Guideline for Clinicians Performing Clinical Studies with Fluorescence Imaging JF Journal of Nuclear Medicine JO J Nucl Med FD Society of Nuclear Medicine SP 640 OP 645 DO 10.2967/jnumed.121.262975 VO 63 IS 5 A1 Wido Heeman A1 Jasper Vonk A1 Vasilis Ntziachristos A1 Brian W. Pogue A1 Rudi A.J.O. Dierckx A1 Schelto Kruijff A1 Gooitzen M. van Dam YR 2022 UL http://jnm.snmjournals.org/content/63/5/640.abstract AB Fluorescence imaging is an emerging imaging technique that has shown many benefits for clinical care. Currently, the field is in rapid clinical translation, and an unprecedented number of clinical trials are performed. Clinicians are inundated with numerous opportunities and combinations of different imaging modalities. To streamline this process, a multidisciplinary approach is needed with drug discovery, software and systems engineering, and translational medicine. Here, we discuss the main constituents of a uniform fluorescence imaging protocol to match the clinical need and ensure consistent study designs and reliable data collection in clinical trials. In an era in which the potential of fluorescence imaging has become evident, consistent conduct of studies, data analysis, and data interpretation is essential for implementation into the standard of care.