TY - JOUR T1 - Semiautomatic Tumor Delineation for Evaluation of <sup>64</sup>Cu-DOTATATE PET/CT in Patients with Neuroendocrine Neoplasms: Prognostication Based on Lowest Lesion Uptake and Total Tumor Volume JF - Journal of Nuclear Medicine JO - J Nucl Med SP - 1564 LP - 1570 DO - 10.2967/jnumed.120.258392 VL - 62 IS - 11 AU - Esben Andreas Carlsen AU - Camilla Bardram Johnbeck AU - Mathias Loft AU - Andreas Pfeifer AU - Peter Oturai AU - Seppo W. Langer AU - Ulrich Knigge AU - Claes Nøhr Ladefoged AU - Andreas Kjaer Y1 - 2021/11/01 UR - http://jnm.snmjournals.org/content/62/11/1564.abstract N2 - Patients with neuroendocrine neoplasms (NENs) have heterogeneous somatostatin receptor expression, with highly differentiated lesions having higher expression. Receptor expression of the total tumor burden may be visualized by somatostatin receptor imaging, such as with 64Cu-DOTATATE PET/CT. Assessment of maximal lesion uptake is associated with progression-free survival (PFS) but not overall survival (OS). We hypothesized that the lesion with the lowest, rather than the highest, 64Cu-DOTATATE uptake would be more prognostic, and we developed a semiautomatic method for evaluating this hypothesis. Methods: Patients with NENs underwent 64Cu-DOTATATE PET/CT. A standardized semiautomatic tumor delineation method was developed and used to identify the lesion with the lowest uptake, that is, with the lowest SUVmean. Additionally, we assessed total tumor volume derived from the semiautomatic tumor delineation. Kaplan–Meier and Cox regression analyses were used to determine whether there was any association with OS and PFS. Results: In 116 patients with NENs, median PFS (95% CI) was 23 mo (range, 20–31 mo) and median OS was 85 mo (range, 68–113 mo). Minimum SUVmean and total tumor volume were significantly associated with PFS and OS in univariate Cox regression analyses, whereas SUVmax was significant only for PFS. In multivariate Cox analyses, both minimum SUVmean and total tumor volume remained statistically significant. Minimum SUVmean and total tumor volume were then dichotomized by their median, and patients were categorized into 4 groups: high or low total tumor volume and high or low minimum SUVmean. Patients with a low total tumor volume and high minimum SUVmean had a hazard ratio of 0.32 (95% CI, 0.20–0.51) for PFS and 0.24 (95% CI, 0.13–0.43) for OS, both with P values of less than 0.001 (reference: high total tumor volume and low minimum SUVmean). Conclusion: We propose a standardized semiautomatic tumor delineation method to identify the lesion with the lowest 64Cu-DOTATATE uptake and total tumor volume. Assessment of the lowest, rather than the highest, lesion uptake greatly increases prognostication by 64Cu-DOTATATE PET/CT. Combining lesion uptake and total tumor volume, we derived a novel prognostic classification system for patients with NENs. ER -