RT Journal Article
SR Electronic
T1 PARP1: A Potential Molecular Marker to Identify Cancer During Colposcopy Procedures
JF Journal of Nuclear Medicine
JO J Nucl Med
FD Society of Nuclear Medicine
SP 941
OP 948
DO 10.2967/jnumed.120.253575
VO 62
IS 7
A1 Paula Demétrio de Souza França
A1 Navjot Guru
A1 Abigail R. Kostolansky
A1 Audrey Mauguen
A1 Giacomo Pirovano
A1 Susanne Kossatz
A1 Sheryl Roberts
A1 Marcio Abrahão
A1 Snehal G. Patel
A1 Kay J. Park
A1 Thomas Reiner
A1 Elizabeth Jewell
YR 2021
UL http://jnm.snmjournals.org/content/62/7/941.abstract
AB Despite efforts in prevention, cervical cancer still presents with a high worldwide incidence and remains a great problem in public health, especially in low-income countries. Screening programs, such as colposcopy with Papanicolaou testing, have greatly improved mortality rates. However, the agents currently used to delineate those lesions (topical application of acetic acid or Lugol iodine) lack specificity and sometimes can lead to unnecessary biopsies or even cervical excisions. A tool to enable in vivo histology to quickly and quantitatively distinguish between tumor, dysplastic tissue, and healthy tissue would be of great clinical interest. Methods: Here, we describe the use of PARPi-FL, a fluorescent inhibitor of poly[adenosine diphosphate-ribose]polymerase 1 (PARP1), which is a nuclear enzyme that is overexpressed in cancer when compared with the normal surrounding tissues. We exploit its use as an optical imaging agent to specifically target PARP1 expression, which was demonstrated to be higher in cervical cancer than the normal surrounding tissue. Results: After topical application of PARPi-FL on freshly excised cone biopsy samples, the nuclei of tumor cells emitted a specific fluorescent signal that could be visualized using a handheld fluorescence confocal microscope. Conclusion: This approach has the potential to improve in vivo identification of tumor cells during colposcopy examination, allowing a rapid, noninvasive, and accurate histopathologic assessment.