Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • Log out
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • Log out
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • View or Listen to JNM Podcast
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Follow JNM on Twitter
  • Subscribe to our RSS feeds
OtherTranslational (Animal and Human)

Combined PARP1-targeted nuclear contrast and reflectance contrast enhances confocal microscopic detection of basal cell carcinoma

Aditi Sahu, Jose Cordero, Xiancheng Wu, Susanne Kossatz, Ucalene Harris, Paula Demetrio Desouza Franca, Nicholas R Kurtansky, Niasia Everett, Stephen Dusza, Jilliana Monnier, Piyush Kumar, Christi Alessi-Fox, Christian Brand, Sheryl Roberts, Kivanc Kose, William Phillip, Erica Lee, Chih-Shan Jason Chen, Anthony Rossi, Kishwer Nehal, Melissa Pulitzer, Caterina Longo, Allan Halpern, Thomas Reiner, Milind Rajadhyaksha and Manu Jain
Journal of Nuclear Medicine October 2021, jnumed.121.262600; DOI: https://doi.org/10.2967/jnumed.121.262600
Aditi Sahu
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jose Cordero
2 University of Puerto Rico - Medical Sciences Campus;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiancheng Wu
3 SUNY Upstate Medical University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susanne Kossatz
4 Technical University of Munich;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ucalene Harris
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paula Demetrio Desouza Franca
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas R Kurtansky
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Niasia Everett
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen Dusza
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jilliana Monnier
5 Aix-Marseille University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Piyush Kumar
6 Icahn School of Medicine at Mt. Sinai;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christi Alessi-Fox
7 Caliber Imaging and Diagnostics;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christian Brand
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sheryl Roberts
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kivanc Kose
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William Phillip
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erica Lee
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chih-Shan Jason Chen
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anthony Rossi
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kishwer Nehal
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Melissa Pulitzer
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caterina Longo
8 University of Modena and Reggio Emilia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Allan Halpern
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Reiner
9 MSKCC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Milind Rajadhyaksha
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Manu Jain
1 Memorial Sloan Kettering Cancer Center, United States;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF
Loading

Abstract

Reflectance confocal microscopy (RCM) with endogenous backscattered contrast can noninvasively image basal cell carcinomas (BCCs) in skin. However, BCCs present with high nuclear density and the relatively weak backscattering from nuclei impose a fundamental limit on contrast, detectability, and diagnostic accuracy. We investigated PARPi-FL, an exogenous nuclear poly (ADP-ribose) polymerase (PARP1)-targeted fluorescent contrast agent and fluorescence confocal microscopy (FCM) towards improving BCC diagnosis. Methods: We tested PARP1 expression in 95 BCC tissues using immunohistochemistry, followed by PARPi-FL staining in 32 fresh surgical BCC specimens. Diagnostic accuracy of PARPi-FL contrast was evaluated in 83 surgical specimens. Optimal parameters for trans-epidermal permeability of PARPi-FL through intact skin was tested ex vivo on 5 human skin specimens and in vivo in 3 adult Yorkshire pigs. Results: We found significantly higher PARP1 expression and PARPi-FL binding in BCCs, as compared to normal skin structures. Blinded reading of RCM-and-FCM images by two experts demonstrated a higher diagnostic accuracy for BCCs with combined fluorescence and reflectance contrast, as compared to RCM-alone. Optimal parameters (time and concentration) for PARPi-FL trans-epidermal permeation through intact skin were successfully determined. Conclusion: Combined fluorescence and reflectance contrast may improve noninvasive BCC diagnosis with confocal microscopy.

  • Molecular Imaging
  • Oncology: General
  • Optical
  • Other
  • cancer diagnosis
  • fluorescence confocal microscopy
  • nuclear contrast
  • optical imaging
  • reflectance confocal microscopy
  • Copyright © 2021 by the Society of Nuclear Medicine and Molecular Imaging, Inc.
PreviousNext
Back to top

In this issue

Journal of Nuclear Medicine: 66 (5)
Journal of Nuclear Medicine
Vol. 66, Issue 5
May 1, 2025
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Complete Issue (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Combined PARP1-targeted nuclear contrast and reflectance contrast enhances confocal microscopic detection of basal cell carcinoma
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
Combined PARP1-targeted nuclear contrast and reflectance contrast enhances confocal microscopic detection of basal cell carcinoma
Aditi Sahu, Jose Cordero, Xiancheng Wu, Susanne Kossatz, Ucalene Harris, Paula Demetrio Desouza Franca, Nicholas R Kurtansky, Niasia Everett, Stephen Dusza, Jilliana Monnier, Piyush Kumar, Christi Alessi-Fox, Christian Brand, Sheryl Roberts, Kivanc Kose, William Phillip, Erica Lee, Chih-Shan Jason Chen, Anthony Rossi, Kishwer Nehal, Melissa Pulitzer, Caterina Longo, Allan Halpern, Thomas Reiner, Milind Rajadhyaksha, Manu Jain
Journal of Nuclear Medicine Oct 2021, jnumed.121.262600; DOI: 10.2967/jnumed.121.262600

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Combined PARP1-targeted nuclear contrast and reflectance contrast enhances confocal microscopic detection of basal cell carcinoma
Aditi Sahu, Jose Cordero, Xiancheng Wu, Susanne Kossatz, Ucalene Harris, Paula Demetrio Desouza Franca, Nicholas R Kurtansky, Niasia Everett, Stephen Dusza, Jilliana Monnier, Piyush Kumar, Christi Alessi-Fox, Christian Brand, Sheryl Roberts, Kivanc Kose, William Phillip, Erica Lee, Chih-Shan Jason Chen, Anthony Rossi, Kishwer Nehal, Melissa Pulitzer, Caterina Longo, Allan Halpern, Thomas Reiner, Milind Rajadhyaksha, Manu Jain
Journal of Nuclear Medicine Oct 2021, jnumed.121.262600; DOI: 10.2967/jnumed.121.262600
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF

Related Articles

  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

Similar Articles

Keywords

  • Molecular imaging
  • Oncology: General
  • optical
  • Other
  • cancer diagnosis
  • fluorescence confocal microscopy
  • nuclear contrast
  • optical imaging
  • reflectance confocal microscopy
SNMMI

© 2025 SNMMI

Powered by HighWire