Research ArticleBasic Science Investigation
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Short-Term Colony-Stimulating Factor 1 Receptor Inhibition–Induced Repopulation After Stroke Assessed by Longitudinal 18F-DPA-714 PET Imaging

Cristina Barca, Amanda J. Kiliaan, Lydia Wachsmuth, Claudia Foray, Sven Hermann, Cornelius Faber, Michael Schäfers, Maximilian Wiesmann, Bastian Zinnhardt and Andreas H. Jacobs
Journal of Nuclear Medicine September 2022, 63 (9) 1408-1414; DOI: https://doi.org/10.2967/jnumed.121.263004

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  • FIGURE 1.
    FIGURE 1.

    Study design. IHC = immunohistochemistry; rt qPCR = real-time quantitative polymerase chain reaction; tMCAo = transient middle cerebral artery occlusion.

  • FIGURE 2.
    FIGURE 2.

    (A) Representative 18F-DPA-714 PET/CT images and corresponding T2-weighted MR images. (B) Quantification of mean 18F-DPA-714 uptake (percentage injected dose/mL) within infarct and contralateral striatum. (C) Infarct-to-contralateral striatum ratio. Treatment: ***P < 0.005. Day 7: ###P < 0.005. Day 30: &P < 0.05, &&&P < 0.005. %ID = percentage injected dose; T2w = T2-weighted.

  • FIGURE 3.
    FIGURE 3.

    Immunofluorescent staining for TSPO, CSF-1R, and CD68 in Iba-1–positive cell population in both control and PLX5622-treated mice on day 14 after ischemia. < = Iba-1–positive cell positive for marker; > = Iba-1–positive cell negative for marker; scale bar = 20 µm. DAPI = 4′,6-diamidino-2-phenylindole.

  • FIGURE 4.
    FIGURE 4.

    Gene expression of microglia- or macrophage-related markers on day 14 after ischemia. Treatment: *P < 0.05, **P < 0.01, ***P < 0.005. Contralateral hemisphere: #P < 0.05, ##P < 0.01, ###P < 0.005. mRNA = messenger RNA.

  • FIGURE 5.
    FIGURE 5.

    Behavioral tests: distance traveled (A), speed in open field (B), latency to fall during rotarod test (C), velocity during pole test (D), and forelimb strength as assessed with grip test (E). Treatment: *P < 0.05, **P < 0.01, ***P < 0.005. Baseline: #P < 0.05, ##P < 0.01, ###P < 0.005. Day 14: &P < 0.05.

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