Abstract
1262
Introduction: Photothermal therapy (PTT) is a novel cancer treatment method, which utilizes heating materials to generate hyperthermia to kill tumor tissues locally by a specific laser irradiation. Prussian blue nanoparticle (PB NPs) is a material that is in the focus as a next generation photothermal treatment material to overcome the low repetition efficiency of existing metal nanoparticles. Bacterial cellulose (BC) is an organic compound produced by certain types of bacteria and has been successfully applied to tissue engineering, drug delivery system, wound dressing, and artificial skin, based on its excellent biocompatibility, flexibility, and porosity. Herein, we developed homogeneously grown PB onto the BC (BC-PB) for the enhanced photothermal therapeutic effect with a high biocompatibility to decrease systematic toxicity of PB NPs.
Methods: BC-PB composites were synthesized based on a bottom-up method as shown in the Figure 1. The purified and fractionated BC was reacted with Cu(OH)2 dissolved NH4OH and 10% HCl mixed solution. After the reaction, the BC was washed with DI water for several times. K3Fe(CN)6 (0.25, 0.5, 0.75, 1 mmol) and 1M HCl was mixed with the comminuted BC for 24 hours. The final BC-PB was characterized by UV-visible spectrophotometer, scanning electron microscope (SEM), 808-nm laser to acquire its absorbance spectrum, morphology, and hyperthermia ability. Cytotoxicity test and In vitro PTT were performed with BC and BC-PB amount series (0, 2, 10, 20, 40, 100 μg/200 μL) in 4T1 breast cancer cell and Raw 264.7 cell lines. The NS, PB NPs, and BC-PB was injected peritumorally in 4T1 tumor bearing mouse for in vivo PTT.
Results: The BC-PB composite had a same absorbance spectrum with PB NPs (λmax: ~780 nm). As the concentration of K3Fe(CN)6 increases, the PB NPs grew on the BC substrate more densely and homogeneously. Temperature increase was proportional to the concentration of PB NPs. The temperature reached about 48℃ at the 1 mmol of PB NPs (ΔT: 23℃) after the irradiation (Figure 2, Supporting figure 1). The BC-PB had almost 80% of cell killing effect more than 20 μg of the BC-PB after the PTT. BC and BC-PB were not toxic without laser irradiation up to a concentration of 100 μg / 200 μL, but PB NPs showed a significant cellular cytotoxicity in Raw 264.7 at a relatively low concentration of 20 μg / 200 μL (Figure 3, Supporting figure 2). The BC-PB with laser irradiation had a significantly higher in vivo hyperthermal therapeutic effect than other controls based on photothermal imaging, tumor volume, and weight changes during the follow up for 18 days (Figure 4, Supporting figure 4).
Conclusions: BC-PB was successfully synthesized where PB NPs was deposited homogeneously and highly packed onto the biocompatible BC substrate. The BC-PB was non-toxic and showed effective therapeutic results in vitro and in vivo PTT tests. As suggested in this study, the BC-PB composite could be a next generation biocompatible PTT platform for an anti-cancer therapeutic strategy.
In this issue
Jump to section
Related Articles
Cited By...
- No citing articles found.