Abstract
3100
Objectives: Positron emission tomography (PET) with high spatial resolution plays a pivotal role for study with human neuronal behavior. In physiologic brain function, including blood flow, metabolism, and neurotransmission, anesthetized animals showed reduced neural activities and brain metabolism. Whether awake or anesthetized during PET imaging, physical stress may reduce neural activities and metabolism, and hence the expected outcome of PET imaging. Therefore meticulous monitoring is vital. Whether in awake or anesthetized animals, physical stress changes the neural activities and metabolism during PET imaging, and to be expected, the outcome of PET imaging. Therefore, careful post-anesthesia monitoring is important and required for reporting. Diet and temperature may influence [F-18]-flurodeoxyglucoe (FDG) uptake of normal tissue, and anesthetics are known to lower body and head temperature, and hence [F-18]-FDG uptake of the brain. In neuroscience study, such as blood flow, metabolism, and neurotransmitter studies, utilization of general anesthesia resulted in different competition effects in primates and humans. In studies regarding cerebral acetylcholinesterase activity, there’s also a difference in radioligand binding between awake and anaesthetized animals. We concluded that general anesthesia poses a great impact on neuronal systems and hence the binding of PET radiotracers and the result of PET imaging. In our plan, we attempt to use the unique adaptation method of our newly constructed animal PET scanner, allowing laboratory animals to be imaged awake in a limited range of motion, to track head motion in freely moving mice and rats during PET acquisitions. True uptake values of radioligands in living and awake mouse brains can be calculated and calibrated using the tracking algorithm and post hoc correction. The uptake of the radiotracer of interest in neuropsychopharmacology can thus be measured in anesthesia-free subjects. In addition, as the cerebral physiology closely correlates with the neural activation and cognitive performance in the brain, it is important to examine the duration effects of isoflurane on CBF and brain functionality when subjects are anesthetized.