Abstract
Radioimmunotherapy of solid tumors using antibody-targeted radionuclides has been limited by low therapeutic indices (TIs). We recently reported a novel 3-step pretargeted radioimmunotherapy (PRIT) strategy based on a glycoprotein A33 (GPA33)–targeting bispecific antibody and a small-molecule radioactive hapten, a complex of 177Lu and S-2-(4-aminobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (177Lu-DOTA-Bn), that leads to high TIs for radiosensitive tissues such as blood (TI = 73) and kidney (TI = 12). We tested our hypothesis that a fractionated anti-GPA33 DOTA-PRIT regimen calibrated to deliver a radiation absorbed dose to tumor of more than 100 Gy would lead to a high probability of tumor cure while being well tolerated by nude mice bearing subcutaneous GPA33-positive SW1222 xenografts. Methods: We treated groups of nude mice bearing 7-d-old SW1222 xenografts with a fractionated 3-cycle anti-GPA33 DOTA-PRIT regimen (total administered 177Lu-DOTA-Bn activity, 167 MBq/mouse; estimated radiation absorbed dose to tumor, 110 Gy). In randomly selected mice undergoing treatment, serial SPECT/CT imaging was used to monitor treatment response and calculate radiation absorbed doses to tumor. Necropsy was done on surviving animals 100–200 d after treatment to determine frequency of cure and assess select normal tissues for treatment-related histopathologies. Results: Rapid exponential tumor progression was observed in control treatment groups (i.e., no treatment or 177Lu-DOTA-Bn only), leading to euthanasia due to excessive tumor burden, whereas 10 of 10 complete responses were observed for the DOTA-PRIT–treated animals within 30 d. Treatment was well tolerated, and 100% histologic cure was achieved in 9 of 9 assessable animals without detectable radiation damage to critical organs, including bone marrow and kidney. Radiation absorbed doses to tumor derived from SPECT/CT (102 Gy) and from biodistribution (110 Gy) agreed to within 6.9%. Of the total dose of approximately 100 Gy, the first dose contributes 30%, the second dose 60%, and the third dose 10%. Conclusion: In a GPA33-positive human colorectal cancer xenograft mouse model, we validated a SPECT/CT-based theranostic PRIT regimen that led to 100% complete responses and 100% cures without any treatment-related toxicities, based on high TIs for radiosensitive tissues. These studies support the view that anti-GPA33 DOTA-PRIT will be a potent radioimmunotherapy regimen for GPA33-positive colorectal cancer tumors in humans.
Radioimmunotherapy has been investigated for decades as a promising therapeutic strategy for selective targeting of ionizing radioisotopes to liquid and solid tumors in humans (1). Few successes in safely and effectively treating tumors by this approach have been achieved in solid tumors (1). An effective radioimmunotherapy approach would address a major unmet need, especially for patients with advanced metastatic colorectal cancer (CRC), a cancer with a 5-y survival of only 11%. Unfortunately, numerous clinical investigations of radioimmunotherapy against CRC-associated antigens have yielded limited clinical benefit, irrespective of radioisotope used (1). The main hurdle observed was insufficient radiation delivery to tumor at radioimmunotherapy activities compatible with body tolerance. Maximal treatment benefits reported thus far using radioimmunotherapy with whole IgG have been stabilized tumor growth in a minority of treated patients, and calculated radiation-absorbed doses have been relatively low; for example, the use of 131I-huA33 to target GPA33 in CRC resulted in modest tumor doses ranging from 12 to 33 Gy at the maximum tolerated dose of 1,480 MBq/m2, without cure in small tumors, and with a corresponding mean red marrow–absorbed dose of approximately 122 cGy (therapeutic index [TI] = ∼16) (2,3).
TI in radioimmunotherapy can be greatly improved using a multistep method, such as the pretargeting approach, based on the discovery of Reardan, et al., that antibodies could be developed against metal chelates (4). This allowed for the separation of the antibody-based tumor-targeting step from the radioactive small molecular hapten component. Additional improvements in targeting to tumor were achieved by modifications, such as the use of streptavidin by Axworthy et al. (5). However, these ultimately failed based on serious practical drawbacks related to immunogenicity of reagents and low TI (1).
In response to these limitations, Orcutt et al. used mathematic modeling to estimate that a hapten-binding antibody must have an affinity of less than 100 pM for efficient delivery of ionizing radiation in pretargeted radioimmunotherapy (PRIT) (6) and developed a novel PRIT approach called DOTA-PRIT (Fig. 1). The primary features include that the modular bispecific antibody format (IgG-scFv) offers multivalent and simultaneous antibody binding of 2 distinct antigens: a tumor antigen and the pM affinity anti-DOTA hapten antibody fragment C825 that recognizes small radioactive yttrium- or lutetium-chelate complexes of DOTA-Bn (6); and the use of radio-haptens (e.g., 177Lu-DOTA-Bn) with almost exclusive renal clearance and with negligible retention in normal tissue (7)).
The purpose of this study was to investigate the efficacy and toxicity of a 3-cycle fractionated DOTA-PRIT regimen capable of delivering an absorbed dose of more than 100 Gy to subcutaneous human CRC tumor xenografts. We report our results with 177Lu, a theranostic isotope that enables noninvasive treatment monitoring and dosimetry in combination with therapeutic β-emission (half-life = 6.7 d; maximum energy of the primary [78.6%] β-emission from 177Lu = 0.5 MeV; average energy of the primary β-emission from 177Lu = 0.13 MeV; maximum range = 1.5 mm). The γ-emissions of 177Lu (208 and 113 keV, with 10% and 6% abundance, respectively) enable direct quantitative theranostic SPECT imaging during treatment. A subaim was to validate the SPECT imaging component of DOTA-PRIT for direct tumor dosimetry, on a treatment cycle–specific basis.
MATERIALS AND METHODS
Reagents and General Procedures
The anti-GPA33 DOTA-PRIT bispecific antibody huA33-C825 (210 kDa) was expressed and purified as described by Cheal et al. (8). A treatment cycle of anti-GPA33 DOTA-PRIT consisted of 3 separate intravenous injections via the tail vein: 0.25 mg of huA33-C825 at −28 h, then 62.5 μg of clearing agent at −4 h, and 55 MBq of 177Lu-DOTA-Bn at 0 (8). The GPA33-postive human CRC cell line SW1222 was obtained from the Ludwig Institute for Cancer Immunotherapy and grown by serial passage. All animal experiments were done in accordance with protocols approved by the Institutional Animal Care and Use Committee of Memorial Sloan Kettering Cancer Center, following National Institutes of Health guidelines for animal welfare. Technical details regarding cell culture and animal models were previously described by Cheal et al. (8).
DOTA-PRIT Regimen
Mice bearing subcutaneous SW1222 xenografts (30–170 mm3, 7 d after inoculation) with either no treatment (n = 5; tumor volume = 114 ± 35 mm3), 177Lu-DOTA-Bn only (n = 5; tumor volume = 128 ± 34 mm3; 2 cycles, total administered 177Lu-DOTA-Bn activity: 110 MBq), or a 3-cycle DOTA-PRIT regimen consisting of anti-GPA33 PRIT + 55.5 MBq of 177Lu-DOTA-Bn (n = 10; tumor volume = 83 ± 38 mm3; total administered 177Lu-DOTA-Bn activity/mouse: 167 MBq) were studied. For DOTA-PRIT, cycle 1 was given on days 7 and 8 after tumor inoculation. This was repeated 6 d later (i.e., weekly), with cycle 2 given on days 14 and 15 and cycle 3 given on days 21 and 22. All groups were monitored 2–3 times per week for overall health, body weight, and measurement of tumor burden by external caliper, with tumor volume calculated using the volume of an ellipsoid. Mice were sacrificed when the tumor volume exceeded 2,500 mm3 (or earlier if the tumor burden interfered with mobility), or if excessive weight loss (>25%) from pretreatment baseline was noted. Tumor growth was analyzed by performing a nonlinear regression fit of an exponential growth curve to the tumor volume data collected in the first 21 d after tumor inoculation for the nontreated and treatment with 177Lu-DOTA-Bn–only groups. The tumor growth data of the animals treated with the 3-cycle DOTA-PRIT regimen were fitted by an initial exponential growth curve up to and including day 14 (i.e., onset time of growth delay and shrinkage) using GraphPad Prism (version 6.00).
Monitoring of DOTA-PRIT with SPECT/CT
All SPECT/CT scans were obtained using a dedicated small-animal scanner (NanoSPECT/CT; Bioscan) with an animal palette heated to 37°C. Animals were anesthetized during scanning using a mixture of 1.5%–2% isoflurane (Baxter Healthcare) and oxygen gas. A CT topogram was acquired first, followed by a 360° small-animal SPECT using a 4-head γ-camera with pinhole collimators (1.4 mm). The SPECT scan time was adjusted for each mouse to record approximately 30,000 counts minimum per frame (20–55 min). Bioscan HiSPECT software was used for iterative image reconstruction and fusion of CT and SPECT images.
Five of ten of the animals undergoing DOTA-PRIT were randomly selected for serial noninvasive SPECT/CT imaging for verification of tumor targeting and calculation of tumor dosimetry. Because of the length of time required for each scan (30–40 min per mouse), the imaging field of view was limited to the caudal half of the animal (midline to tail). Each animal was imaged 5–6 times at various time points during fractionated treatment, up to 24 h after injection of cycle 3 with 177Lu-DOTA-Bn (day 23) (Fig. 2). For each image, tumor volumes were estimated by CT image analysis, and the total activity in the tumor region was estimated by SPECT image analysis. Tumor count rates were converted to activity concentrations (MBq per mm3 or gram [g]) using the measured system calibration factor for 177Lu.
To determine whether partial-volume correction would be necessary, a phantom consisting of 4 fillable spheres with diameters of 5.8, 7.8, 9.9, and 12.4 mm was imaged on the SPECT/CT with a solution of 177Lu having a nominal concentration of 3.7 MBq (0.1 mCi)/mL. Regions of interest were drawn on the coregistered CT images and activity within the spheres measured in the reconstructed SPECT images. A diameter-dependent recovery curve was fitted and compared with the CT-measured tumor sizes. A partial-volume effect was not observed in the phantom SPECT images above a size of approximately 200 mm3. For the measured tumor sizes, we estimated a minimum recovery coefficient of 0.86 for cycle 1 and 0.71 for cycle 3. Because most tumor sizes were greater than about 200 mm3 for the duration of the experiment, we did not apply a partial-volume correction to the results.
SPECT Dosimetry
177Lu-DOTA-Bn activity concentration was measured by SPECT/CT imaging of the SW1222 xenograft mice. Activity concentrations as a function of time without decay correction for cycles 1 and 2 were fit to a single exponential. The apparent half-life for this exponential therefore incorporated both physical and biologic decay effects.
Durational cumulated activity was calculated for each cycle from 0 to 160 h after the start of cycle. Durational cumulated activity for cycle 2 therefore includes residual activity from cycle 1. Activity for cycle 3 was extrapolated by assuming an exponential with the same effective half-life as cycle 2. This assumption is based on the fact that cycle 3 should behave similarly to cycle 2 as both start while there is residual activity still present from the previous treatment cycle. Total cumulated activities were also calculated for each cycle from the start of the multicycle protocol, assuming no further treatment and complete elimination of the tumor at 496 h after injection of 177Lu activity in cycle 1. Total cumulated activity for cycle 1 represents dose delivered from 1 injection from 0 to 496 hours after injection. Total cumulated activity for cycle 2 represents dose delivered from the first and second injections (but not the third) assuming the same rate of decay for cycle 2 to 496 h.
Tumor time–activity curves were also simulated for the 3-cycle treatment regimen. Curve parameters from the exponential fit to the first cycle of each subject were used to generate the corresponding second and third cycle curves. Residual activity from each previous cycle was taken into account in the initial activity at the beginning of each cycle. Elimination of radiolabel was assumed to proceed at the same exponential rate as for the first cycle. Mean tumor-absorbed doses were calculated as the product of the cumulated activities thus determined and the average energy emitted per decay of 177Lu in the form of β-particles, assuming complete local absorption of the β-particles and ignoring the dose contribution of the much more penetrating γ-rays.
Necropsy of Animals That Underwent DOTA-PRIT
Mice were euthanized with CO2 according to the guidelines of the American Veterinary Medical Association. After gross examination, organs were fixed in 10% neutral buffered formalin. After fixation, bones were decalcified in a formic acid solution (Surgipath Decalcifier I; Leica Biosystems). Fixed organs were further processed for histology in ethanol and xylene in a Leica ASP6025 tissue processor and embedded in paraffin. Paraffin blocks were sectioned at 5 μm, stained with hematoxylin and eosin, and examined by a board-certified veterinary pathologist. The following tissues were examined: skin and subcutaneous tissue from tumor implantation site, liver, kidney, spleen, and bone marrow (sternum, vertebrae, femur, and tibia). Any abnormal gross or histologic findings were noted.
Hematology and Serum Chemistry
For hematology, blood was collected into tubes containing EDTA. Automated analysis was performed by the LCP on an IDEXX Procyte DX hematology analyzer, and the following parameters were determined: white blood cell count, red blood cell count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red blood cell distribution width standard deviation and coefficient of variance, platelet count, platelet distribution width, mean platelet volume, and relative and absolute counts of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and reticulocytes.
For serum chemistry, blood was collected into tubes containing a serum separator and then centrifuged to obtain serum for analysis. Serum chemistry was performed by the LCP on a Beckman Coulter AU680 analyzer for the following parameters: alkaline phosphatase concentration, alanine aminotransferase concentration, aspartate aminotransferase concentration, creatine kinase concentration, gamma-glutamyl transpeptidase concentration, albumin concentration, total protein concentration, globulin concentration, albumin/globulin ratio, total bilirubin concentration, blood urea nitrogen concentration, creatinine concentration, cholesterol concentration, triglycerides concentration, glucose concentration, calcium concentration, phosphorus concentration, chloride concentration, potassium concentration (K), sodium concentration (Na), Na/K ratio.
Statistical Analysis
Kaplan–Meier survival analysis or statistical comparisons between groups (Student t test) were performed with Prism software (version 6.0; GraphPad Software). For all studies, the level of statistical significance is set at a P level of less than 0.05.
RESULTS
Fractionated Treatment with DOTA-PRIT
No significant difference in average tumor volumes was found among the 3 groups at the start of treatment. Tumor sizes measured over the course of the experiment demonstrated a clear difference in efficacy between the control treatment groups (i.e., no treatment or 177Lu-DOTA-Bn only) and the DOTA-PRIT–treated groups. This is shown in Figure 3A where tumor volumes are plotted as a function of time after xenograft inoculation for all treatment groups.
Kaplan–Meier plots (Fig. 3B) were generated for all 3 groups. Development of a 1,000 mm3 tumor volume that necessitated euthanasia due to excessive tumor burden was designated as the survival endpoint. All untreated mice and mice treated with 177Lu-DOTA-Bn only (10/10, 100%) developed large, exponentially growing tumors, requiring euthanasia of the animals. Individual tumor doubling times ranged from 3.6 to 6.0 d (R2 = 0.90–0.99). Because of this rapid tumor growth, treatment with 177Lu-DOTA-Bn only was limited to just 2 cycles. The median time to a terminal 1,000 mm3 tumor volume was 19 d for no treatment and 16 d for treatment with 2 cycles of 177Lu-DOTA-Bn only. For all DOTA-PRIT–treated mice (10/10, 100%), the tumors grew exponentially to a maximum size of 247–386 mm3 on day 14 (tumor doubling time range, 2.2–5.1 d; R2 = 0.82–0.99). After the third cycle of treatment (administered on days 21 and 22, after tumor inoculation), tumors showed rapid regression to below the palpable threshold (≤10 mm3) by approximately day 30 (Fig. 3A). Tumor volumes at the start of cycles 2 and 3 were 298 ± 51 mm3 and 31 ± 19 mm3, respectively, measured manually by caliper. For the DOTA-PRIT–treated mice, median time to the terminal 1,000 mm3 tumor burden was never reached because all animals (10/10) showed complete responses with no recurrence before study endpoints (118–200 d after inoculation). By log-rank (Mantel–Cox) test, survival of the DOTA-PRIT group was significant (P < 0.0001).
SPECT/CT Imaging to Monitor Treatment and Quantify Tumoricidal Tumor Doses
SPECT/CT of tumor-bearing mice undergoing treatment demonstrated highly selective tumor localization of pretargeted 177Lu-DOTA-Bn as early as 1 h after injection of 177Lu-DOTA-Bn. At this time, the average effective 177Lu activity concentration in tumors (as average MBq/g ± SD; n = 4) was 3.92 ± 0.85 MBq/g, corresponding to an uptake of injected 177Lu-DOTA-Bn of 7.1% ± 1.5% per g of tumor volume. Prolonged and high tumor retention of 177Lu activity was also observed at 24 and 160 h after injection (non–decay-corrected activities, 3.17 ± 1.10 and 0.64 ± 0.19 MBq/g), suggesting minimal biologic clearance of 177Lu activity from the tumor. Activity in the bladder region is apparent in the SPECT images at 1 h after injection, consistent with renal clearance of free 177Lu-DOTA-Bn (data not shown). Rapid renal clearance of free 177Lu-DOTA-Bn was also supported by the observation that whole-body activity of each individual animal, determined by assay in a dose calibrator, was similar to the measured tumor-associated activity. 177Lu activity remaining in the mice measured after 24 and 160 h after injection of cycle 1 (as average ± SD; n = 5) was 6.98% ± 1.64% and 1.18% ± 0.39% of administered activity, respectively. Although the SPECT/CT imaging demonstrates that the most prominent focus of activity was in the tumor, low levels of 177Lu activity were detected in the liver, spleen, and kidney (∼10- to 15-fold-less activity than tumor, as percentage injected dose per gram of tissue, based on previous biodistribution studies). For cycle 2, the whole-body percentage of administered 177Lu activity remaining after 24 and 160 h after injection was 12.83% ± 2.01% and 1.98% ± 0.44%, respectively. Finally, for cycle 3, the whole-body percentage of administered 177Lu activity remaining after 24 h was 4.81% ± 1.03% (no additional data were collected for cycle 3 after 24 h).
The CT image–derived tumor volumes (in mm3) demonstrating tumor doubling followed by regression are shown in Table 1. SPECT image–derived 177Lu activity estimates for tumor regions of interest are shown in Table 2 and Figure 4. Tumor dosimetry was calculated from 177Lu time–activity curves and is presented in Table 3. Representative serial SPECT/CT images originating from the same animal are provided in Figure 5. The contribution to total dose was estimated to be greatest during cycle 2 (average dose during cycle 2 period, 43.8 Gy; n = 5). The fitted exponential decay curves of the 3-cycle treatment regimen are shown in Figure 6, illustrating the difference between the measured activity and expected activity from single and 2-cycle protocols. Total dose from the 3-cycle treatment was estimated at 102 Gy. Radiation absorbed doses to tumors derived from SPECT/CT (102 Gy) and biodistribution (109.6 Gy) agreed to within 6.9%. For comparison, 177Lu activity in the tumor for a 3-cycle treatment was simulated on the basis of kinetics derived only from the first cycle of the experimental data. The time–activity curves are shown in Supplemental Figure 1 (supplemental materials are available at http://jnm.snmjournals.org), with tumor dose estimates calculated from the curves shown in Supplemental Table 1. Total tumor dose predicted by the simulation was about 92 Gy, assuming first-cycle kinetics throughout, that is, the kinetics of subsequent cycles essentially ignore the effects of previous cycles. Measured dose from the 3-cycle treatment was higher than predicted.
Toxicity of Fractionated DOTA-PRIT
All treatments were well tolerated, with no average weight loss greater than 10% from baseline observation (Supplemental Fig. 2), suggesting no acute toxicity. For DOTA-PRIT–treated mice, a slight drop in average weight (∼5%) from baseline was observed for the duration of treatment administration, but was reversible to pretreatment weight about 10 d after the final 177Lu-DOTA-Bn injection. Weight loss was most likely the result of a transient and reversible gastrointestinal toxicity, which did not leave permanent histologic changes, but formal toxicity studies are warranted to verify this. No early, treatment-related deaths were observed.
Histopathology confirmed complete tumor disappearance in all surviving DOTA-PRIT–treated mice (9/9) at 3–6 mo (96–178 d) after treatment. No evidence of tumors was observed macroscopically or histologically at the site of tumor inoculation or in distant organs in 9 of 9 mice that underwent necropsy, consistent with histologic complete cures in those animals (100%).
We found no evidence of treatment-related radiotoxicity in radiosensitive organs in treated animals at either 3 or 6 mo after treatment. For example, in the 5 mice submitted for necropsy and histopathology assessment at 3 mo after treatment, no abnormalities, such as bone marrow depletion as seen in 177Lu-IgG radioimmunotherapy (9), nephrotoxicity, or increased incidence of mesangial glomerulopathy as seen with 177Lu-peptide receptor radiotherapy (10), were observed.
A variety of histopathologic changes were noted; these are presented in Supplemental Table 3. Although most of these findings were compatible with naturally occurring background lesions typical in mice of this strain and age, 2 lesions observed with a low incidence were not typical spontaneous lesions. These 2 lesions, for which a link to test article–induced toxicity could not be ruled out, were mild to moderate granulomatous hepatitis or splenitis (2/9 mice) and marked pulmonary interstitial fibrosis (1/9 mice). Sudden deterioration or death was observed in 2 of 10 treated animals 136 and 158 d after treatment. In 1 animal, this was determined to be due to pulmonary fibrosis of undetermined cause (as mentioned previously) with secondary myocardial hypertrophy; the other mouse could not be analyzed because of the condition of the animal when it was discovered.
DISCUSSION
We herein report a 3-cycle, curative theranostic regimen with minimal toxicity for SW1222, a human solid CRC xenograft, derived from a patient with a moderately well-differentiated adenocarcinoma of the colon (classification, Dukes’ stage C, a locally invasive cancer). There have been extensive preclinical studies with multistep regimens, but the outcome we report, of a high probability (100%) of histologic cures without detectable radiosensitive organ toxicity, is to the best of our knowledge, extremely unusual. Our preclinical development focus has been on hitting therapeutic benchmarks, which must be achieved in human translational trials as well. These benchmarks include, for example, cumulative doses to solid tumor of more than 100 Gy and TIs for radiosensitive tissues of more than 12 for the kidneys, 70 for bone marrow, and 40 for colon. We chose these benchmarks based on clinical human normal-tissue radiation dose tolerance estimates (11), as well as experience gleaned from radiopeptide treatments and related toxicities in humans (1).
In our preliminary studies with anti-GPA33 DOTA-PRIT and SW1222, dose escalation resulted in progressive tumor response, and that toxicity was not limiting (8). At lower tumor doses (∼40–70 Gy), treatment led to complete responses (e.g., we observed 4/4 complete responses for a 2-cycle treatment regimen of 55.5 MBq per cycle) but was ultimately subcurative and resulted in 2 distinct recurrence phenotypes: outgrowth of antigen-positive tumor with the same rate of growth as controls (seen in 3/4); and a histologic senescence pattern that resulted in dormant tumor, in which antigen-expressing cell growth arrested after 1–2 doublings (seen in 1/4; observed at 140 d after injection). It is critical to assess long-term outcome after radioimmunotherapy, because we and others (12) have shown that late recurrence can occur in this model, with relatively high frequency when treatment is suboptimal. In this study, we found that repeated dose cycles to higher tumor doses were required to achieve 100% histologic cures in 9 of 9 treated animals available for assessment by necropsy. With the 3-dose regimen, we achieved our targets and in this CRC model, we obtained cures with minimal toxicity (mean TIs for tumor–to–normal tissue were ∼73 [blood], ∼10 [liver], ∼10 [spleen], ∼12 [kidney], and ∼110 [marrow-bearing bone (femur)] when 10,000 cGy were delivered to tumor; Tables 1–3 provide dose estimates).
DOTA-PRIT should provide an ideal theranostic platform that permits the use of a single radiopharmaceutical entity for both quantitative imaging and therapy, by simply changing the amount of radioactivity administered. The use of noninvasive SPECT has the advantage of providing a tumor radiation estimate for all 3 doses in the regimen, which is otherwise impossible to achieve by biodistribution (i.e., terminal) studies alone. The absorbed doses to tumor, 10,160 ± 141 cGy, were estimated using SPECT. We defined and derived the dose contribution from each cycle by calculating the total predicted dose after that cycle and subtracting what would have been the total dose, had treatment stopped after the previous cycle. When these contributions as proportions of the total cumulated dose after 3 treatment cycles until total regression of the tumor at 496 h are considered, it turns out that 60% of the dose is from the second cycle, and cycles 1 (30%) and 3 (10%) contribute smaller fractions. This result is nonintuitive but is based on dynamic measured changes in tumor volume expansion, which in turn is based on CT during cycle-1 treatment, followed by rapid regression during cycle 2 in particular (Table 1).
As a novel feature of this work, we determined that the addition of cycle 3 contributed 10% of the total absorbed dose by tumor, which was essential for histologic cure of the 9 of 9 tumors examined after treatment. This result may relate to the state of the treated tumor at the time the third cycle is added. Such information is crucial for later treatment planning in the clinic and underscores the importance of a multiple-cycle treatment regimen that is closely monitored with theranostics.
CONCLUSION
This study validated a theranostic curative radionuclide therapy regimen with no clear evidence of radiation-induced toxicity in a murine model bearing human CRC solid-tumor xenografts. We demonstrated that 3 cycles were required to achieve tumor doses of more than 100 Gy, and with TIs of greater than 70 for blood and greater than 10 for kidney we observed no radiation-related toxicity despite high total administered 177Lu activity (167 MBq/mouse). Cycle-specific tumor dosimetry was demonstrated using serial SPECT/CT of animals undergoing treatment. Collectively, these preclinical studies support clinical translation of anti-GPA33 DOTA-PRIT.
DISCLOSURE
This study was supported in part by the Donna & Benjamin M. Rosen Chair (to Steven M. Larson), Enid A. Haupt Chair (to Nai-Kong V. Cheung), The Center for Targeted Radioimmunotherapy and Theranostics, Ludwig Center for Cancer Immunotherapy of Memorial Sloan Kettering Cancer Center (to Steven M. Larson), and R01-CA101830 (K. Dane Wittrup). Steven M. Larson was also supported in part by P50-CA86438. We also acknowledge the NIH/NCI Cancer Center Support Grant (P30 CA008748) for use of the Tri-Institutional Laboratory of Comparative Pathology, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, NY. Technical services provided by the Memorial Sloan Kettering Small-Animal Imaging Core Facility were also supported by Cancer Center Support Grant P30 CA08748 (to C. Thompson). A Shared Resources Grant from the Memorial Sloan Kettering Cancer Center Metastasis Research Center (to Pat B. Zanzonico), which provided funding for the purchase of the NanoSPECT/CT Plus, is gratefully acknowledged. Steven M. Larson, Nai-Kong V. Cheung, and K. Dane Wittrup own stock in Voreyda Theranostics, Inc. Additionally, Sarah M. Cheal, Hong Xu, Steven M. Larson, and Nai-Kong V. Cheung are inventors on an international patent application for “Multi-Specific Antibodies with Affinity for Human A33 Antigen and DOTA Metal Complex and Uses Thereof,” filed on February 9, 2016. No other potential conflict of interest relevant to this article was reported.
Acknowledgments
We gratefully acknowledge Blesida Punzalan for her technical assistance. We are also grateful to Leah Bassity for her editorial assistance.
Footnotes
Published online Jul. 13, 2017.
- © 2017 by the Society of Nuclear Medicine and Molecular Imaging.
REFERENCES
- Received for publication March 13, 2017.
- Accepted for publication May 31, 2017.