Abstract
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Objectives Hepatocarcinoma is the most common cancer in China. To explore a more effective approach for the treatment of hepatocarcinoma, we transfected the recombinant lentivirus E8-codA-GFP into HepG2 cells and investigate the killing effect on the infected HepG2 cells under the radiation of 125I.
Methods The recombinant lentivirus E8-codA-GFP was constructed and packaged and infected HepG2 cells. Then the infected cells were exposed to 125I at different dose and the expression of GFP gene was observed. The killing effects were performed by MTT assay when cells were exposed to 125I at different radioactivities combined with 400µg/ml 5-FC and the cells were exposed to the same dose of 125I only as negative contrast and exposed to 400µg/ml 5-FU as positive contrast.
Results After the lentivirus-infected HepG2 cells were exposed to different doses of 125I for 72 hours, green fluorescence can be obviously observed, especially at the dose of 185kBq. MTT colorimetric assay showed that the survival rates of lentivirus-infected HepG2 cells treated with 125I combined with 400µg/ml 5-FC were lower than that of the control groups treated with the same dose of 125I only but a little higher than the positive contrast group treated with 400µg/ml 5-FU(Tab.1).
Conclusions The results indicate that synthetic promoter E8 can induce the expression of downstream gene after exposure of 125I, and non-toxic 5-FC combined with low dosage 125I can play certain killing effects on infected HepG2 cells. These results provide valuable bases to the further experiments on low dosage radionuclide combination with CD/5-FC suicide system in the therapy of. hepatocarcinoma.
Research Support This work was supported by the grants from Beijing Natural Science Foundation (No. 7083115 and 7112129) and Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine (No. KF201101).