Article Figures & Data
Figures
- FIGURE 1.
(A) Photomicrographs of brain sections from animals at days 10, 14, 21, and 28 after intracerebral injection of 4T1-GFP cells. Reactive astrocytes were identified by GFAP immunoreactivity (brown stain), and sections were counterstained with cresyl violet. Scale bar = 100 μm. (B) Significant positive correlation was found between volume of astrocyte activation and tumor volume (y = 18.2x + 0.415; r2 = 0.657; P < 0.0001). (C) No significant differences were found in ratio of astrocyte to tumor volume over time (n = 4–5 per group).
- FIGURE 2.
(A) Photomicrographs of brain sections from a naive BALB/c mouse and at days 10, 14, and 28 after intracardiac injection of 4T1-GFP cells. Reactive astrocytes were identified by GFAP immunoreactivity (brown stain), and sections were counterstained with cresyl violet. Scale bar = 100 μm. (B) Significant positive correlation was found between extent of astrocyte activation and tumor area (y = 11x + 0.006; r2 = 0.6; P < 0.0001). (C) Significant increase in ratio of astrocyte to tumor area was found over time (*P < 0.05, n = 5–6 per group). (D) Photomicrographs comparing microglial infiltration of metastases, detected by Iba-1 immunoreactivity, with astrocyte activation (brown stain in each case). Graph shows quantitation of microglial (black bars) and astrocyte (gray bars) activation at days 10 (tumor number = 16), 14 (n = 39), and 28 (n = 7). *P < 0.05. ***P < 0.0001.
- FIGURE 3.
Confocal microscopy images obtained from mouse brain 14 d after intrastriatal injection of 4T1-GFP cells (A) or 21 d after intracardiac injection (B) demonstrating TSPO colocalization with either astrocyte reactivity (GFAP) surrounding metastases or reactive microglia in tumor periphery (IBA-1). Scale bar = 50 μm.
- FIGURE 4.
(A) Representative SPECT images at injection site (left hemisphere) from 4T1-GFP–injected mouse and saline-injected control. (B) 123I-DPA713 binding was confirmed by autoradiography in 4T1-GFP–injected animals as compared with saline-injected animals. (C–D) Graphs show percentage signal increase in injected hemisphere, compared with control contralateral hemisphere for 4T1-GFP– (n = 6) and saline- (n = 3) injected animals for either SPECT (C) or autoradiography (D) data. *P < 0.05.
- FIGURE 5.
Representative autoradiography image from mouse brain obtained 21 d after intracardiac injection of 4T1-GFP cells; areas of increased signal correlated spatially with immunohistochemical detection of tumor burden, as seen in immunohistochemistry image. Graph shows quantification of 123I-DPA713 binding (normalized activity), as determined by SPECT, in animals injected intracardially with either 4T1-GFP cells or saline.
Additional Files
Supplemental Data
Files in this Data Supplement:
