Abstract
1182
Objectives this study was to investigate whether asiaticoside affect against 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinogenicity in vitro (MCF-7 cells) and in vivo (DMBA-induced rat cancer).
Methods MTT assay using H2O2 alone and H2O2+different asiaticoside concentrations was performed on MCF-7 cells for 48 h. Flow cytometry, caspases 3 and the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1) were quantified. 99mTc-MIBI (MIBI) imaging was performed on rats divided into five groups designated I (control), II (DMBA-induced cancer), III (pre- and post- treatment with asiaticoside (200 µg/animal) in DMBA-induced cancer), IV (post- treatment with asiaticoside in DMBA-induced cancer), and V (treated with asiaticoside alone, drug control). Twelve weeks post-DMBA, rats developed mammary tumors. Animals either were sacrificed or imaged with MIBI. Histological examination on tumor tissues was done. Tumor MIBI uptake ratios were determined by drawing region of interest over tumor divided by whole body counts. Data was expressed as the mean±standard deviation. Appropriate t-test and anova statistical methods were used to compare data.
Results The IC50 of asiaticoside for MCF-7 cells was determined as 40 µM. Asiaticoside potential of the cytotoxicity of hydrogen peroxide and the caspase-3 activity for MCF-7 cells for 48 h was increasing with asiaticoside dose. The cytokines of TNF-α and IL-1β expression significantly decreased and correlated with MIBI uptake ratios in vitro and in vivo after asiaticoside administration.
Conclusions This study showed that asiaticoside is effective in vitro and in vivo in inducing apoptosis, enhancing anti-tumor activity, significantly reducing tumor volumes, tumor necrosis, TNF-α and IL-1β cytokinin and MIBI uptake ratios.
Research Support Grant number MN01/09.