Abstract
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Objectives To explore the temporal expression of Tenascin-C, that transiently expressed in area with active tissue remodeling under various pathologic conditions, rat heart with ischemia and reperfusion was imaged using I-125-labeled anti-Tenascin-C-antibody (TenC-Ab).
Methods The left coronary artery was occluded for 30-min followed by reperfusion for 1, 3, 7, 14, and 28 days, and I-125-TenC-Ab (2 MBq) was injected intravenously. 4-5 hr later Tc-99m-MIBI (150 MBq) was injected intravenously just after the left coronary artery reocclusion to verify the area at risk and the rats were sacrificed. Dual tracer autoradiography was performed to assess uptake ratio (UR) of TenC-Ab.
Results 1 and 3 day after reperfusion, significant uptake of the I-125-TenC-Ab was inhomogeneously observed in the area at riak (UR=2.99±0.90 and 2.71±0.80, respectively). 7 and 14 days after reperfusion, the uptake decreased (UR=1.94±0.23 and 2.06±0.37, respectively), followed by weak uptake after 28 days of reperfusion (UR=1.47±0.27). Immuno-histochemical analysis revealed that the TenC-Ab accumulates around infracted areas.
Conclusions In conclusion, I-125-TenC-Ab imaging may be a novel way to monitor the myocardial injury and its repair process.
- © 2009 by Society of Nuclear Medicine