Abstract
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Objectives C-11 choline and C-11 acetate are promising PET tracers for prostate cancer evaluation. However, the molecular basis of increased choline and acetate uptake in prostate cancer has not been comprehensively assessed. We examined the RNA transcriptomes of prostate cancer vs. normal prostate using microarray approaches with special emphasis on choline and acetate metabolic pathways.
Methods 69 cancer and 20 normal prostate tissues transcriptomes from prostate cancer study (GSE6956) was selected from the public GEO dataset. Affy U133A 2.0 chips were used (14,500 well characterized genes). After RMA normalization, Spotfire software analysis was performed. Each volume change of major metabolic pathways was estimated with Chi square test using Stata software.
Results Comparing gene profiles of cancer with normal tissues, 10 out of 37 probesets in choline metabolism were up, and 3 probesets down in the cancer group. The balance of the change shifted to increase the volume. 7 of 34 fatty acid synthesis probesets in prostate cancer were up and 1 down. In TCA pathway, 5 out of 54 probesets were up and 6 down in prostate cancer.
Conclusions The transcriptomes of prostate cancer show significant increases in the choline metabolism and fatty acid synthesis pathways vs normal prostate cells. These findings are likely causal for the high accumulation of radiolabeled acetate and choline in this type of cancer in patients as imaged by PET.
- © 2009 by Society of Nuclear Medicine