Abstract
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Objectives: So far, information on feasibility of Aβ-plaque µ-PET imaging in animal models of Alzheimer’s disease (AD) is limited. Aim of this study was to compare the single-transgenic (APPswe) mouse model Tg2576 and a new double-transgenic (APP/PS1) mouse model ARTE10 regarding their suitability for µ-PET imaging.
Methods: Comparable groups of female Tg2576 (n=4, 24.7±1 months) and female ARTE10 (n=4, 22.8±0.3 months) transgenic mice and age matched female wild type (WT) animals were examined. Following inhalation anaesthesia, animals were injected with 10.0-28.7 MBq [11C]PIB in a tail vein and scanned for 60 min p.i. (FOCUS F120/Siemens Inveon). For anatomically correct ROI-placement PET-images were overlaid with MR-data of the animals. The cerebellum was used as a reference region and cortical/cerebellar (ctx/ce) uptake ratios were calculated.
Results: In Tg2576 animals higher ctx/ce-ratios were detected compared to controls. However the differences (mean values: Tg2576 1.11±0.13, WT 1.00±0.09) did not reach statistical significance (p-values>0.05). In contrast, significantly higher ctx/ce-ratios (p-values<0.05) were observed in ARTE10 mice compared to WT (mean values: ARTE10 1.42±0.22, WT 0.97±0.08). Direct comparison between ratios in Tg2576 and ARTE10 revealed significantly higher values in ARTE10 (p-values<0.05). No significant differences between WT-groups was found.
Conclusions: The current study indicates that the feasibility of rodent Aβ-plaque imaging may strongly depend on the animal model used. The double-transgenic ARTE10 model may show superior properties for imaging with [11C]PIB compared to the single-transgenic Tg2576 model. This may be due to differences in quantity and type of amyloid plaque deposition.
- Society of Nuclear Medicine, Inc.