Prevention of radiolysis of monoclonal antibody during labeling

MC Chakrabarti, N Le, CH Paik… - The Journal of …, 1996 - search.proquest.com
17. Khawli LA. Kassis Al. m-['*']lodoaniline: a useful reagent for radiolabeling biotin. Page 1
15. Rosebrough SP. Plasma stability and pharmacokinetics of radiolabeled deferoxaminebiotin …

Protein and peptide labeling with (cyclopentadienyl) tricarbonyl rhenium and technetium

TW Spradau, JA Katzenellenbogen - Bioconjugate chemistry, 1998 - ACS Publications
A method for labeling proteins and peptides with (methoxycarbonyl cyclopentadienyl)
tricarbonyl rhenium and technetium is described. The precursors used for this labeling are …

Can a cysteine challenge assay predict the in vivo behavior of 99mTc-labeled antibodies?

DJ Hnatowich, F Virzi, M Fogarasi… - Nuclear medicine and …, 1994 - Elsevier
Recent investigations have shown that transchelation to cysteine is a principal mode of in
vivo instability of 99m Tc-labeled antibodies. In this investigation, a cysteine challenge assay …

In vitro investigations of tumor targeting with 99mTc-labeled antisense DNA

YM Zhang, Y Wang, N Liu, ZH Zhu… - Journal of Nuclear …, 2001 - Soc Nuclear Med
One objective of this investigation was to determine whether chemical modifications of
oligonucleotides to permit radiolabeling with γ-or positron emitters interferes with …

Technetium-99m labelling of the anti-tumour antibody PR1A3 by photoactivation

MA Stalteri, SJ Mather - European journal of nuclear medicine, 1996 - Springer
Irradiation of antibody with ultraviolet light leads to reduction of disulphide bonds. Thus
irradiation can be used to generate free thiols prior to direct labelling of antibody with …

Micromethod for quantification of SH groups generated after reduction of monoclonal antibodies

NI Escobar, A Morales, G Núñez - Nuclear medicine and biology, 1996 - Elsevier
A simple, rapid, and reproducible micromethod for quantification of sulfhydryl (SH) groups
generated after reduction of monoclonal antibody (MAb) disulfide bonds with 2 …

Water-soluble phosphines for direct labeling of peptides with technetium and rhenium: Insights from electrospray mass spectrometry

WEP Greenland, PJ Blower - Bioconjugate chemistry, 2005 - ACS Publications
Direct labeling of salmon calcitonin (sCT) is possible in one step using water-soluble
phosphines (sulfonated triphenylphosphines) as the reducing agent both for disulfide and …

Direct 99mTc-labeling of antibodies by sodium dithionite reduction, and role of ascorbate as a stabilizer in cysteine challenge

P Qi, SN Muddukrishna, R Torok-Both, J Rahn… - Nuclear medicine and …, 1996 - Elsevier
A method for the direct 99mTc-labeling of antibodies by dithionite reduction was developed.
Among three murine monoclonal IgG1 and one human polyclonal IgG (hIgG) antibodies …

[BOOK][B] Application of solution protein chemistry to biotechnology

RL Lundblad - 2009 - taylorfrancis.com
Reflecting the versatility of the author's science and the depth of his experience, Application
of Solution Protein Chemistry to Biotechnology explores key contributions that protein …

Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique

SN Muddukrishna, A Chen, P Qi… - Applied radiation and …, 1995 - Elsevier
The interference of stannous in the quantitation of sulfhydryl in pretreated MAb using the
Ellman's method is described. A HPLC method which effectively overcomes the interference …