Abstract
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Objectives Positron emission tomography (PET) is a powerful molecular imaging technique that used in the clinic research of human body. Recently, a miniaturized device has been developed as a promising tool for PET imaging, and has been used for radiosynthesis of PET tracers. However, small scale radiosynthesis on micro fluidic devices need to be further studied to evaluate its usefulness. Our objective was to find a more efficient method of microscale radiosynthesis of [18F]FDG, one of the most widely used radiopharmaceutical, by analyzing several reaction conditions for [18F]FDG radiosynthesis.
Methods The radioynthesis of [18F]FDG was carried out using mannose triflate between Teflon-glass substrates (Fig. A and C), which have the same geometry of EWOD chip, and then the crude product was purified via cartridge purification. In this study, we investigated several factors such as reaction solvents, bases, and concentration on the fluorination efficiency.
Results We found that 2,3-dimethyl-2-butanol is a useful solvent for [18F]FDG synthesis. (Fig. H). Based on the studies of several effects on the radio-synthetic reaction, [18F]FDG was successfully synthesized on a micro-scale level with a radiochemical yield of 71% in a relatively shorter time.
Conclusions The small scale of radioynthesis of [18F]FDG was successfully optimized using a protic solvent. The results provide a potentially new method for [18F]FDG radiosynthesis on a microfluidic device and is applicable for PET imaging.